文献类型： 外文期刊

作者： Zhang, Danhua ¹ ; Lin, Runmao ³ ; Yamamoto, Naoki ² ; Wang, Zhaoyilin ⁴ ; Lin, Hui ² ; Okada, Kazunori ⁵ ; Liu, Yao ⁶ ; Xiang, Xing ² ; Zheng, Tengda ² ; Zheng, Haoxi ⁷ ; Yi, Xiaoqun ² ; Noutoshi, Yoshiteru ⁸ ; Zheng, Aiping ¹ ;

作者机构： 1.State Key Lab Crop Gene Explorat & Utilizat Southw, Chengdu, Peoples R China

2.Sichuan Agr Univ, Sch Agron, Chengdu, Peoples R China

3.Hainan Univ, Key Lab Green Prevent & Control Trop Plant Dis & P, Minist Educ, Haikou, Peoples R China

4.Sichuan Agr Univ, Rice Res Inst, Chengdu, Peoples R China

5.Univ Tokyo, Agrobiotechnol Res Ctr, Tokyo, Japan

6.Sichuan Acad Agr Sci, Crop Res Inst, Key Lab Sichuan Prov, Chengdu, Peoples R China

7.Chengdu Foreign Language Sch, Chengdu, Peoples R China

8.Okayama Univ, Grad Sch Environm Life & Nat Sci & Technol, Okayama, Japan

关键词： cell death; effector protein; immune responses; Rhizoctonia solani AG-1 IA; virulence

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.9 ）

ISSN： 1464-6722

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Rhizoctonia solani AG-1 IA causes a necrotrophic rice disease and is a serious threat to rice production. To date, only a few effectors have been characterized in AG-1 IA. We previously identified RsIA_CtaG/Cox11 and showed that infiltration of the recombinant protein into rice leaves caused disease-like symptoms. In the present study, we further characterized the functionality of RsIA_CtaG/Cox11. RsIA_CtaG/Cox11 is an alternative transcript of cytochrome c oxidase copper chaperone Cox11 that starts from the second AUG codon, but contains a functional secretion signal peptide. RNA interference with RsIA_CtaG/Cox11 reduced the pathogenicity of AG-1 IA towards rice and Nicotiana benthamiana without affecting its fitness or mycelial morphology. Transient expression of the RsIA_CtaG/Cox11-GFP fusion protein demonstrated the localization of RsIA_CtaG/Cox11 to mitochondria. Agro-infiltration of RsIA_CtaG/Cox11 into N. benthamiana leaves inhibited cell death by BAX and INF1. In contrast to rice, agro-infiltration of RsIA_CtaG/Cox11 did not induce cell death in N. benthamiana. However, cell death was observed when it was coinfiltrated with Os_CoxVIIa, which encodes a subunit of cytochrome c oxidase. Os_CoxVIIa appeared to interact with RsIA_CtaG/Cox11. The cell death triggered by coexpression of RsIA_CtaG/Cox11 and Os_CoxVIIa is independent of the leucine-rich repeat receptor kinases BAK1/SOBIR1 and enhanced the susceptibility of N. benthamiana to AG-1 IA. Two of the three evolutionarily conserved cysteine residues at positions 25 and 126 of RsIA_CtaG/Cox11 were essential for its immunosuppressive activity, but not for cell death induction. This report suggests that RsIA_CtaG/Cox11 appears to have a dual role in immunosuppression and cell death induction during pathogenesis.