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Fine mapping of Pm58 from Aegilops tauschii conferring powdery mildew resistance

文献类型： 外文期刊

作者： Xue, Shulin 1 ; Hu, Shanshan 1 ; Chen, Xian 1 ; Ma, Yuyu 1 ; Lu, Mingxue 1 ; Bai, Shenglong 1 ; Wang, Xintian 1 ; Sun, Tiepeng 1 ; Wang, Yingxue 1 ; Wan, Hongshen 2 ; An, Xia 3 ; Li, Suoping 1 ;

作者机构： 1.Henan Univ, Coll Agr, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475004, Henan, Peoples R China

2.Sichuan Acad Agr Sci, Crop Res Inst, Key Lab Wheat Biol & Genet Improvement Southweste, Chengdu 610066, Sichuan, Peoples R China

3.Zibo Acad Agr Sci, Zibo 255000, Shandong, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 5 期

页码：

收录情况： SCI

摘要： Key message The powdery mildew resistance gene Pm58 was traced to a 141.3-kb interval with the co-segregating marker Xkasp68500 in wheat breeding. Pm58 is a powdery mildew resistance gene identified in Aegilops tauschii accession TA1662 and effective in a common wheat background. To finely map Pm58, an F-2 population of 676 plants derived from the cross T093 x TA1662 was used for recombinant screening. We obtained 13 recombinants that occurred between the flanking markers Xhnu670 and Xhnu186. Genotyping and phenotyping these recombinant F-2:3 families delimited Pm58 to a 0.22-cM interval (Xsts20220-Xkasp61553) on chromosome arm 2DS. The region carrying the Pm58 locus was approximately 141.3-kb, which contained eight annotated genes according to the reference genome sequence of Ae. tauschii AL8/78. Haplotype analysis of 178 Ae. tauschii accessions using the candidate gene-specific markers identified a disease resistance gene AET2Gv20068500 as a candidate for Pm58. Comparative mapping of the Pm58-containing interval revealed two presence/absence variations (PAVs) between AL8/78 and common wheat Chinese Spring. PAV-1 resides in the 3 '-end of AET2Gv20068500. The majority of 158 common wheat cultivars (84.8%) displayed the absence of a 14.1-kb fragment in the PAV-1 region, which was confirmed by aligning the targeted genome sequences of the other sequenced Ae. tauschii accessions and common wheat cultivars. A co-segregating marker Xkasp68500 developed from AET2Gv20068500 can distinguish TA1662 from all randomly selected common wheat cultivars and will be instrumental for tracking Pm58 in breeding programs.

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