文献类型： 外文期刊

作者： Tay, Yi Juin ² ; Han, Mingming ¹ ; Zou, Zihan ¹ ; Kuang, Xu ⁴ ; Liu, Guoxing ³ ; Jiang, Qichen ³ ; Xiong, Sen ⁵ ; Liang, Ji ¹ ; Abdullah, Anisah Lee Binti ¹ ;

作者机构： 1.Univ Sains Malaysia, Minden 11800, Penang, Malaysia

2.Newcastle Univ, Human Nutr Res Ctr, Newcastle Upon Tyne, England

3.Freshwater Fisheries Res Inst Jiangsu Prov, Low Temp Germplasm Bank Important Econ Fish, Jiangsu Prov Sci & Technol Resources Agr Germplasm, Coordinat Serv Platform, Nanjing, Peoples R China

4.Sichuan Acad Agr Sci, Fisheries Res Inst, Chengdu, Peoples R China

5.Chengdu Jncon Environm Protect Technol Co Ltd, Chengdu, Sichuan, Peoples R China

关键词： Nano-plastics; Immunosuppressive; Hepatopancreas; Antioxidative defense

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.4； 五年影响因子：2.8 ）

ISSN： 1744-117X

年卷期： 2025 年 56 卷

页码：

收录情况： SCI

摘要： Global pollution by microplastics (MPs) and nano-plastics (NPs) disrupts aquatic ecosystems and compromises the health of aquatic animals. However, there is a significant gap in the literature regarding acute high-dose and chronic low-dose polystyrene nano-plastic (PS-NP) exposures that simulate point-source and non-point source pollution events, respectively. The PS-NP concentrations (10 mg/L for acute exposure and 1 mg/L for chronic exposure) were designed to simulate realistic environmental scenarios. In this study, the giant freshwater prawn (Macrobrachium rosenbergii) was used as a model to investigate the differences on physiological functions under acute (96 h, 10 mg/L) and chronic (30 days, 1 mg/L) PS-NP exposure. Transcriptome sequencing and basic biochemical detection were utilized to analyze changes in gene expression, antioxidative and immunological responses of hepatopancreas. The results demonstrated that acute exposure to PS-NPs induced a significant upregulation of antioxidative and pro-inflammatory enzymes, suggesting a rapid stress response and activation of immediate defense mechanisms. In contrast, chronic exposure led to downregulation of mitochondrial and immune pathways, collapse of antioxidative defense, increased lipid peroxidation, and signs of immunosuppression. These findings underscored the time-dependent toxico-dynamics of PS-NPs and highlighted their potential ecological risks under prolonged exposure. The study provided molecular-level evidence to support more accurate environmental risk assessments and informs mitigation strategies for safeguarding aquaculture health in contaminated freshwater ecosystems.