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Functional defect at the rice choline monooxygenase locus from an unusual post-transcriptional processing is associated with the sequence elements of short-direct repeats

文献类型： 外文期刊

作者： Luo, Di 1 ; Niu, Xiangli 1 ; Wang, Yuguo 1 ; Zheng, Wenjing 2 ; Chang, Lijuan; Wang, Qilin; Wei, Xin; Yu, Guiron 1 ;

作者机构： 1.Sichuan Univ, Coll Life Sci, Minist Educ Key Lab Bioresource & Ecoenvironm, Chengdu 610064, Peoples R China; Fudan Univ, Minist Educ, Key Lab Biodivers Sci & Ecol Engn, Inst Biodivers Sci, Shanghai 200433, Peoples R China; Sichuan Acad Agr Sci, Inst Biotechnol, Chengdu 610066, Peoples R China; Sichuan Univ, State Key Lab Hydraul & Mt River Engn, Chengdu 610064, Peoples R China

2.Sichuan Univ, Coll Life Sci, Minist Educ Key Lab Bioresource & Ecoenvironm, Chengdu 610064, Peoples R China; Fudan Univ, Minist Educ, Key Lab Biodivers S

关键词： alternative splicing;choline monooxygenase;glycine betaine;Oryza sativa (rice);short-direct repeats

期刊名称：NEW PHYTOLOGIST （ 影响因子：10.151； 五年影响因子：10.475 ）

ISSN： 0028-646X

年卷期： 2007 年 175 卷 3 期

页码：

收录情况： SCI

摘要： Glycine betaine (GB), a quaternary ammonium solute, plays a crucial role in developing osmotic tolerance. Rice contains a choline monooxygenase (CMO) and two betaine aldehyde dehydrogenase homologues that are required for GB synthesis, but usually no GB is accumulated in rice (Oryza sativa). To elucidate the molecular processes that underlie the GB deficiency in rice, an experiment involving rice and spinach (Spinacia oleracea) was conducted to analyze the products transcribed from CMO genes. Reverse transcription- polymerase chain reaction (RT-PCR) was used to obtain CMO transcripts and a sequencing approach was employed to analyze the structural composition of various CMO transcripts. The results showed that most rice CMO transcripts were processed incorrectly, retaining introns or deleted of coding sequences; the unusual deletion events occurred at sequence elements of the short-direct repeats. In conclusion, the production of incorrect CMO transcripts results in a deficiency of the full-length CMO protein and probably reduces GB accumulation considerably in rice plants. Sequence comparison results also implied that the unusual deletion-site selection might be mediated by the short-direct repeats in response to stress conditions.

  • 相关文献

[1]An unusual posttranscriptional processing in two Betaine aldehyde dehydrogenase loci of cereal crops directed by short, direct repeats in response to stress conditions. Zheng, Wenjing,Lu, Bao-Rong,Ren, Guangjun,Huang, Weizao,Wang, Songhu,Liu, Junli,Tang, Zizhi,Luo, Di,Wang, Yuguo,Liu, Yongsheng.

[2]Short, direct repeats (SDRs)-mediated post-transcriptional processing of a transcription factor gene OsVP1 in rice (Oryza sativa). Niu, Xiangli,Wang, Yuguo,Ren, Guangjun,Zhuo, Ting,Yang, Yi,Lu, Bao-Rong,Liu, Yongsheng. 2007

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