文献类型: 外文期刊
作者: Liu, Y. 1 ; Paul, V. H. 1 ;
作者机构: 1.Fachsch Sudwestfahlen, Labor Biotechnol & Qualitatssicherung, Soest, Germany
关键词: formation of ascospores;germination;sclerotia;Sclerotinia sclerotiorum
期刊名称:JOURNAL OF PLANT DISEASES AND PROTECTION ( 影响因子:1.928; 五年影响因子:1.652 )
ISSN: 1861-3829
年卷期: 2007 年 114 卷 1 期
页码:
收录情况: SCI
摘要: Sclerotial germination of 33 isolates of Sclerotinia sclerotiorum, which were collected from different regions and crops, was compared at 10 degrees C in a climate chamber. Germination of S. sclerotiorum, measured as the number of sclerotia producing stipes and apothecia, was the predominant mode that was affected significantly by isolate. Isolates which originated from a warmer area generally germinated more quickly. The culture media affected the viability of sclerotia of isolates M01 and MZ. Sclerotia germinated on different substrates such as standard gardener's soil, sand and vermiculite, but did not germinate well when the sclerotia had developed on nutrient-rich or nutrient-poor medium. The optimum medium for sclerotial development was wheat grain. Sclerotia produced on wheat grain were well-suited for studies on germination of sclerotia and formation of apothecia. The production of apothecia was optimal at a temperature of 10 degrees C and an illumination of 500 lux, and after an incubation period of 2 to 3 months. one apothecium produced over 1 million ascospores and survived over 15 days in the climate chamber.
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