Characterisation and immunostimulatory activity of an alpha-(1 -> 6)-D-glucan from the cultured Armillariella tabescens mycelia
文献类型： 外文期刊
作者： Luo, Xia 1 ; Xu, Xiaoyan 2 ; Yu, Mengyao 1 ; Yang, Zhirong 1 ; Zheng, Linyong 1 ;
作者机构： 1.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
2.Sichuan Inst Chinese Mat Med, Chengdu 610041, Peoples R China
3.Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Peoples R China
关键词： Armillariella tabescens;polysaccharide;macrophage;nitric oxide;cytokines
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2008 年 111 卷 2 期
页码：
收录情况： SCI
摘要： IPS-B2, an intracellular polysaccharide with anti-tumor effect, was isolated from cultured Armillariella tabescens mycelia by hot water extraction, anion-exchange and gel chromatography. Based on the results of paper chromatography (PC), gas chromatography (GC), infra-red (IR) spectroscopy and (13)C NMR, IPS-B2 was characterized as an alpha-(1 -> 6)-D-glucan with a molecular weight of 49.5 kDa. The effects of IPS-B2 on murine peritoneal macrophages were further investigated. The results demonstrated that IPS-B2 induced nitric oxide (NO) and cytokines (TNF-alpha, IL-1 beta and IL-6) production in macrophages. The outcomes of real-time reverse transcription-polymerase chain reaction (RT-PCR) proved that the transcribing level of inducible NO synthase (iNOS), TNF-alpha, IL-1 beta and IL-6 mRNA in the peritoneal macrophages have been augmented by IPS-B2. These data suggest that the anti-tumor activity of the polysaccharide from A. tabescens may due to activation of macrophage. (C) 2008 Elsevier Ltd. All rights reserved.
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