Amplified fragment length polymorphism (AFLP) and genealogy analysis of the introgressed lines from Gossypium hirsutum x Gossypium barbadense varieties
文献类型： 外文期刊
作者： Liu, Zhao-hui 2 ; Zhang, Yong 1 ; Peng, Jinhua 1 ; Deng, Kejun 1 ; Dong, Shunwen 2 ; Ren, Zhenglong 1 ;
作者机构： 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China
2.Sichuan Acad Agr Sci, Ind Crop Breeding & Cultivat Inst, Jianyang 641400, Peoples R China
3.Sichuan Agr Univ, Key Lab Plant Breeding & Genet, Yaan 625014, Peoples R China
关键词： Cotton;introgressed line;AFLP;genealogical analysis
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2010 年 9 卷 23 期
页码：
收录情况： SCI
摘要： In this study, amplified fragment length polymorphism (AFLP) technique was used to analyze the genealogical relationship of 7 introgressed cotton varieties from the hybridization of upland (Gossypium hirsutum L) and sea-island cottons (Gossypium barbadense L). Ten pairs of primer combinations with high polymorphism and resolution were selected from 64 primers. These 10 primer combinations resulted in a total of 480 bands, of which 374 bands (77.9%) were polymorphic and 51 bands (10.6%) were specific. Cluster analyses showed that 7 varieties of cottons were divided into two groups. Our results suggested that interspecific hybridization is feasible to broaden germplasm of upland cotton and AFLP technique could be applied for the identification of variety purity and genealogical relationship.
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