Genetic diversity analysis of various red spider mite-resistant upland cotton cultivars based on RAPD
文献类型： 外文期刊
作者： Liu, Zhaohui 2 ; Zhang, Yong 1 ; Zhang, Shuo 1 ; Deng, Kejun 1 ; Dong, Shunwen 2 ; Ren, Zhenglong 1 ;
作者机构： 1.Univ Elect Sci & Technol China, Sch Life Sci & Technol, Chengdu 610054, Peoples R China
2.Sichuan Acad Agr Sci, Ind Crop Breeding & Cultivat Inst, Jianyang 641400, Peoples R China
3.Sichuan Agr Univ, Key Lab Plant Breeding & Genet, Yaan 625014, Peoples R China
关键词： Cotton;red spider mite;RAPD;genetic diversity;cluster analysis
期刊名称：AFRICAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.573； 五年影响因子：0.794 ）
ISSN： 1684-5315
年卷期： 2011 年 10 卷 18 期
页码：
收录情况： SCI
摘要： This study is carried out to analyze the genetic diversity of red spider mite-resistant cotton (Gossypium hirsutum) cultivars that are applied in cultivar identification and breeder's right protection of cottons. The genomic DNA was used as template and random primers were used to analyze the genetic diversity of 21 accessions of Gossypium hirsutum by RAPD-PCR. Among the 100 primers screened, 20 primers could generate 176 fragments, 96.02% of which were polymorphic ones. The similarity coefficient of cultivars was between 0.2273 - 0.9773. The average values of effective number of alleles, Nei's gene diversity and Shannon's information index were 1.7391, 0.4017, and 0.5773, respectively. Cluster analysis based on UPGMA revealed that 21 cultivars could be divided into three groups. The analysis revealed that it was corresponding to the geographical distribution and most of the lines had a wide genetic base.
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