文献类型： 外文期刊

作者： Wen, G. Q. ¹ ; Li, J. ¹ ; Liu, X. H. ¹ ; Zhang, Y. S. ¹ ; Wen, S. S. ² ;

作者机构： 1.China West Normal Univ, Coll Life Sci, Nanchong, Peoples R China

2.Sichuan Acad Agr Sci, Rice & Sorghum Res Inst, Deyang, Peoples R China

关键词： Improved cetyltrimethylammonium bromide method;Dioscorea opposita Thunb.;Random amplified polymorphic DNA;Orthogonal design

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 1 期

页码：

收录情况： SCI

摘要： Dioscorea opposita Thunb. has been used as health food and herbal medicinal ingredients in traditional Chinese medicine. In this study, the total DNA of D. opposita Thunb. was extracted using an improved cetyltrimethylammonium bromide (CTAB) method, and the extracted DNA was further used for random amplified polymorphic DNA (RAPD) reaction system by design of the L-16 (4(4)) orthogonal diagram. The results showed that the improved CTAB method can be used to isolate high-quality and high-concentration DNA, and the optimized protocol can overcome the instability of RAPD reaction system. The knowledge stated here can be used to study the genetic diversity of D. opposita Thunb.