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Intra- and inter-isolate variation of ribosomal and protein-coding genes in Pleurotus: implications for molecular identification and phylogeny on fungal groups

文献类型： 外文期刊

作者： He, Xiao-Lan 1 ; Li, Qian 1 ; Peng, Wei-Hong 1 ; Zhou, Jie 1 ; Cao, Xue-Lian 1 ; Wang, Di 1 ; Huang, Zhong-Qian 1 ; Tan, 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Peoples R China

2.Jilin Agr Univ, Changchun 130118, Peoples R China

3.Mianyang Inst Agr Sci, Mianyang 621023, Peoples R China

关键词： Edible mushroom;Intra-isolate polymorphism;Specific variation

期刊名称：BMC MICROBIOLOGY （ 影响因子：3.605； 五年影响因子：4.283 ）

ISSN： 1471-2180

年卷期： 2017 年 17 卷

页码：

收录情况： SCI

摘要： Background: The internal transcribed spacer (ITS), RNA polymerase II second largest subunit (RPB2), and elongation factor 1-alpha (EF1 alpha) are often used in fungal taxonomy and phylogenetic analysis. As we know, an ideal molecular marker used in molecular identification and phylogenetic studies is homogeneous within species, and interspecific variation exceeds intraspecific variation. However, during our process of performing ITS, RPB2, and EF1a sequencing on the Pleurotus spp., we found that intra-isolate sequence polymorphism might be present in these genes because direct sequencing of PCR products failed in some isolates. Therefore, we detected intra-and inter-isolate variation of the three genes in Pleurotus by polymerase chain reaction amplification and cloning in this study. Results: Results showed that intra-isolate variation of ITS was not uncommon but the polymorphic level in each isolate was relatively low in Pleurotus; intra-isolate variations of EF1a and RPB2 sequences were present in an unexpectedly high amount. The polymorphism level differed significantly between ITS, RPB2, and EF1a in the same individual, and the intra-isolate heterogeneity level of each gene varied between isolates within the same species. Intra-isolate and intraspecific variation of ITS in the tested isolates was less than interspecific variation, and intra-isolate and intraspecific variation of RPB2 was probably equal with interspecific divergence. Meanwhile, intra-isolate and intraspecific variation of EF1a could exceed interspecific divergence. These findings suggested that RPB2 and EF1a are not desirable barcoding candidates for Pleurotus. We also discussed the reason why rDNA and protein-coding genes showed variants within a single isolate in Pleurotus, but must be addressed in further research. Conclusions: Our study demonstrated that intra-isolate variation of ribosomal and protein-coding genes are likely widespread in fungi. This has implications for studies on fungal evolution, taxonomy, phylogenetics, and population genetics. More extensive sampling of these genes and other candidates will be required to ensure reliability as phylogenetic markers and DNA barcodes.

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