Cloning and characterization of Agp1, the gene encoding the small subunit of ADP-glucose pyrophosphorylase from wheat and its relatives
文献类型： 外文期刊
作者： Zhang, Xiao-Wei 1 ; Wang, Qing 2 ; Zhang, Ling-Ling 1 ; Zhong, Xiao-Juan 1 ; Jiang, Qian-Tao 1 ; Ma, Jian 1 ; Qi, Peng 1 ;
作者机构： 1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Agr Univ, Coll Agron, Chengdu 611130, Sichuan, Peoples R
关键词： ADP-glucose pyrophosphorylase small subunit;cDNA sequence;functional analysis;gene structure;glycogen synthesis
期刊名称：BIOLOGIA （ 影响因子：1.35； 五年影响因子：1.139 ）
ISSN： 0006-3088
年卷期： 2017 年 72 卷 12 期
页码：
收录情况： SCI
摘要： ADP-glucose pyrophosphorylase (AGP) consists of two large (AGP-L) and two small (AGP-S) subunits; it regulates the limiting step in the biosynthesis of starch. Here, we isolated the full-length cDNA sequence of the AGP-S gene (designed as Agp1) from wheat and whole genomic sequences from relatives of wheat. The coding region of Agp1 ranged from 5948 bp to 7666 bp and contained 9 exons and 8 introns. The full-length cDNA sequence of Agp1 encoded a polypeptide containing 473 amino acids without transit peptides. The sequence alignment and phylogenetic tree analyses indicated that the Agp1 cDNA sequence was highly conserved (99.7%) across the diploid Triticeae species examined in this study. Variations in introns caused the Agp1 genomic sequences from wheat relatives to be less similar (79.5%), and Agp1 sequences from the B genome donor species were divergent. In developing grains, rapid increase in Agp1 expression at prophase was closely followed by the accumulation of starch, and a significant (P < 0.001) correlation was observed between them. The heterogeneous mixtures of small and large subunits from different wheat species restarted glycogen synthesis in the Escherichia coli AC70R1-504 cells, which are deficient in endogenous AGP activity (glg C-/-). Our data provide useful information to evaluate and utilize Agp1 sequences in Triticeae species.
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