Identification of male sex-specific markers using genome re-sequencing in the Chinese longsnout catfish Leiocassis longirostris
文献类型： 外文期刊
作者： Luo, Hui 1 ; Li, Yu 1 ; Zheng, Shuqing 2 ; Zhou, Jian 4 ; Zou, Xinxi 1 ; Li, Chuangju 5 ; Ye, Huan 5 ; Li, Zhe 1 ; Zhou, Chaowei 1 ; Lv, Guangjun 1 ; Xiao, Shijun 6 ; Ye, Hua 1 ;
作者机构： 1.Southwest Univ, Coll Fisheries, Key Lab Freshwater Fish Reprod & Dev, Minist Educ, Chongqing 402460, Peoples R China
2.Key Lab Aquat Sci Chongqing, Chongqing 400175, Peoples R China
3.Aquat Sci & Technol Innovat Alliance Chongqing, Chongqing 402460, Peoples R China
4.Sichuan Acad Agr Sci, Fisheries Inst, Chengdu 611731, Peoples R China
5.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr & Rural Affairs, Wuhan 430223, Peoples R China
6.Jiaxing Key Lab New Germplasm Breeding Econ Mycol, Jiaxing 314000, Zhejiang, Peoples R China
关键词： Leiocassis longirostris; Sex-specific marker; Sex chromosome; Sex determining region
期刊名称：AQUACULTURE （ 影响因子：5.135； 五年影响因子：5.125 ）
ISSN： 0044-8486
年卷期： 2022 年 558 卷
页码：
收录情况： SCI
摘要： The Chinese longsnout catfish (Leiocassis longirostris Gunther), an indigenous commercially important fish species in China, exhibits markedly sex dimorphism in growth, which the males grow much faster than females. So, we aimed to develop sex-specific DNA markers to accelerate the development of sex control techniques for L. longirostris. In this study, 15 individuals of each sex were sequenced using whole-genome re-sequencing technology. A total of 238.51 Gb clean data was used to detect the sex associated markers and we found that 2472 loci were enriched in the first 18 Mb of Chr 7, the putative sex-determination region for L. longirostris. All candidate markers were male heterozygous, implying that the sex-determination system of the L. longirostris is the XX/XY type. Using the sequence polymorphism of male and female individuals in this region, we designed six pairs of primers to develop sex-specific markers, of which three markers exclusively exhibited male-specific bands. Further validation showed that the accuracy of sex prediction was up to 100% for individuals in the Meishan, Chengdu, Yibin and Shishou regions, suggesting wide applicability among local populations. Importantly, marker 3 was located in the ACAP1 gene, which may be involved in sex determination or differentiation of L. longirostris. This method for developing sex-specific markers is efficient, simple, and provides a useful reference that can be applied to other fish species. The markers developed in this work could be instrumental for sex control breeding to increase the yield in the L. longirostris aquaculture industry.
- 相关文献
作者其他论文 更多>>
-
Fertilization type affects the genetic potential for phosphorus mineralization but not for phosphorus solubilization at the continental scale
作者：Yuan, Yanhong;Ren, Chengjie;Barquero, Maria Belen;Bastida, Felipe;Li, Yu;Zhang, Shuiqing;Fan, Hongzhu;Zhang, Huimin;Li, Qiang;Liu, Shutang;Shi, Linlin;Shi, Linlin;Cai, Andong;Xu, Minggang;Xu, Hu
关键词：Fertilization; Phosphorus; Solubilization; Mineralization; Metagenomes
-
Full-Length Transcriptome Sequencing and hsp Gene Family Analysis Provide New Insights into the Stress Response Mechanisms of Mystus guttatus
作者：Qin, Lang;Zhang, Xueling;Li, Yu;Luo, Hui;Ye, Hua;Zhang, Xueling;Luo, Hui;Ye, Hua;Zhang, Xueling;Li, Yusen;Shi, Jun;Han, Yaoquan;Wang, Dapeng;Lin, Yong
关键词：full-length transcriptome; stress response mechanisms; heat shock protein;
Mystus guttatus -
Stress Response of Siniperca chuatsi to Transport Stimuli Using Compound Feed and Live Bait
作者：Duan, Yuanliang;Li, Qiang;Huang, Zhipeng;Zhao, Zhongmeng;Zhao, Han;Feng, Yang;Liu, Senyue;Mou, Chengyan;Zhou, Jian;Zhang, Lu;Zhou, Jian;Zhang, Lu
关键词：Siniperca chuatsi; stress response; compound feed; antioxidant capacity; transport stimuli
-
OsMATE7-mediated flavonol accumulation regulates pollen tube growth in rice
作者：Fan, Shijun;Huang, Kailai;Yang, Yan;Xi, Yi;Ye, Huan;Xu, Lei;Chen, Weilan;Gao, Peng;Li, Ting;Tu, Bin;Yuan, Hua;Ma, Bingtian;Wang, Yuping;Zhong, Zhaohui;Xiong, Jiawei;Kang, Liangzhu;Tang, Shiwen;Chen, Xuewei;Li, Shigui;Qin, Peng;Fan, Shijun;Xiang, Chengbin;Gao, Peng
关键词：rice; MATE protein; pollen tube growth; flavonols transport; starch hydrolysis
-
Sex Differences in the Growth of Channel Catfish Revealed by Analyzing the Relationship Between Morphological Traits and Body Mass
作者：Xie, Bingjie;Chen, Xiaohui;Duan, Yongqiang;Liu, Hongyan;Zhong, Liqiang;Wang, Minghua;Chen, Xiaohui;Zhang, Shiyong;Duan, Yongqiang;Liu, Hongyan;Zhong, Liqiang;Wang, Minghua;Chen, Xiaohui;Zhang, Shiyong;Tang, Luyu;Chen, Xiaohui;Zhang, Shiyong;Mou, Chengyan;Zhou, Jian
关键词：Body mass; Channel catfish; Morphological traits; Multiple regression equation; Sex
-
Early brain gene network alterations and growth disruptions in juvenile Chinese longsnout catfish (Leiocassis longirostris) induced by 17(3-estradiol and 17α-methyltestosterone
作者：Feng, Yang;Deng, Yongqiang;Zhao, Zhongmeng;Zhao, Han;Liu, Senyue;Zhang, Lu;Duan, Yuanliang;Huang, Zhipeng;Du, Jun;Li, Qiang;Zhou, Jian;Mou, Chengyan;Ye, Hua
关键词：17(3-estradiol; 17 alpha-methyltestosterone; Chinese longsnout catfish ( Leiocassis; Brain; Immediate early genes
-
Gene-metabolite correlations underpin the hepatic fibrosis and metabolic disruption induced by methyltestosterone in juvenile Chinese longsnout catfish (Leiocassis longirostris)
作者：Feng, Yang;Deng, Yongqiang;Li, Zhao;Zhao, Zhongmeng;Zhao, Han;Liu, Senyue;Zhang, Lu;Duan, Yuanliang;Huang, Zhipeng;Du, Jun;Li, Qiang;Zhou, Jian;Mou, Chengyan;Wei, Zheng
关键词：Methyltestosterone; Chinese longsnout catfish(Leiocassis longirostris); Liver toxicity; Fibrosis; Lipid metabolism disruption; Multi-omics integration
