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Thidiazuron Enhances Strawberry Shoot Multiplication by Regulating Hormone Signal Transduction Pathways

文献类型： 外文期刊

作者： Wang, Fang 1 ; Li, Yali 2 ; Pang, Yadan 1 ; Hu, Jiangtao 1 ; Kang, Xinna 4 ; Qian, Chun 3 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China

2.Sichuan Acad Agr Sci, Inst Remote Sensing & Digital Agr, Chengdu 610066, Peoples R China

3.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400712, Peoples R China

4.Shijiazhuang Acad Agr & Forestry Sci, Shijiazhuang 050080, Peoples R China

关键词： thidiazuron (TDZ); shoot multiplication; strawberry (Fragaria x ananassa); hormone signal transduction; transcriptome analysis

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 9 期

页码：

收录情况： SCI

摘要： Tissue culture-based rapid propagation is critical for genetic improvement and virus-free production of strawberries (Fragaria x ananassa). This study evaluated the optimal concentration of thidiazuron (TDZ) for shoot multiplication and explored the underlying molecular mechanisms. Strawberry explants were treated with TDZ at concentrations of 0, 0.025, 0.05, 0.1, and 0.4 mgL-1 in vitro, and growth, physiological changes, and transcriptomic profiles were analyzed after four weeks. The results identified 0.05 mgL-1 TDZ as the most effective concentration for shoot proliferation, yielding a significant increase in leaf number. However, TDZ application inhibited plant height and reduced chlorophyll, carotenoid, and soluble sugar contents. Physiological analyses revealed that TDZ decreased endogenous cytokinin levels while elevating auxin concentrations. Transcriptomic analysis showed that TDZ suppressed cytokinin biosynthesis and up-regulated cytokinin oxidase expression, thereby modulating hormone homeostasis. Additionally, TDZ enhanced the cytokinin signaling pathway, which is crucial for cell division and shoot initiation, and influenced auxin, gibberellin, and brassinosteroid pathways to regulate differentiation. These findings suggest that TDZ promotes strawberry shoot multiplication primarily through hormone signal transduction, providing insights for optimizing tissue culture protocols.

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