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Characterization and Differentiation of Grain Proteomes from Wild-Type Puroindoline and Variants in Wheat

文献类型： 外文期刊

作者： Liu, Peixun 1 ; Liu, Zehou 1 ; Ma, Xiaofei 2 ; Wan, Hongshen 1 ; Zheng, Jianmin 1 ; Luo, Jiangtao 1 ; Deng, Qingyan 1 ; Mao, Qiang 1 ; Li, Xiaoye 1 ; Pu, Zongjun 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Crop Res Inst, Environm Friendly Crop Germplasm Innovat & Genet I, Key Lab Wheat Biol & Genet Improvement Southwester, Chengdu 610066, Peoples R China

2.Shanxi Agr Univ, Wheat Res Inst, Linfen 041000, Peoples R China

关键词： Triticum aestivum; grain hardness; proteomics; puroindoline genotypes

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

ISSN：

年卷期： 2023 年 12 卷 10 期

页码：

收录情况： SCI

摘要： Premium wheat with a high end-use quality is generally lacking in China, especially high-quality hard and soft wheat. Pina-D1 and Pinb-D1 (puroindoline genes) influence wheat grain hardness (i.e., important wheat quality-related parameter) and are among the main targets in wheat breeding programs. However, the mechanism by which puroindoline genes control grain hardness remains unclear. In this study, three hard wheat puroindoline variants (MY26, GX3, and ZM1) were compared with a soft wheat variety (CM605) containing the wild-type puroindoline genotype. Specifically, proteomic methods were used to screen for differentially abundant proteins (DAPs). In total, 6253 proteins were identified and quantified via a high-throughput tandem mass tag quantitative proteomic analysis. Of the 208 DAPs, 115, 116, and 99 proteins were differentially expressed between MY26, GX3, and ZM1 (hard wheat varieties) and CM605, respectively. The cluster analysis of protein relative abundances divided the proteins into six clusters. Of these proteins, 67 and 41 proteins were, respectively, more and less abundant in CM605 than in MY26, GX3, and ZM1. Enrichment analyses detected six GO terms, five KEGG pathways, and five IPR terms that were shared by all three comparisons. Furthermore, 12 proteins associated with these terms or pathways were found to be differentially expressed in each comparison. These proteins, which included cysteine proteinase inhibitors, invertases, low-molecular-weight glutenin subunits, and alpha amylase inhibitors, may be involved in the regulation of grain hardness. The candidate genes identified in this study may be relevant for future analyses of the regulatory mechanism underlying grain hardness.

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