Identification of the MADS-Box Gene Family and Development of Simple Sequence Repeat Markers in Chimonanthus praecox
文献类型： 外文期刊
作者： Wu, Huafeng 1 ; Liu, Bin 1 ; Cao, Yinzhu 1 ; Ma, Guanpeng 1 ; Zheng, Xiaowen 1 ; Yang, Ximeng 3 ; Dai, Qianli 3 ; Zhu, Hengxing 3 ; Zhu, Haoxiang 4 ; Song, Xingrong 5 ; Sui, Shunzhao 1 ;
作者机构： 1.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing Engn Res Ctr Floriculture,Minist Educ, Key Lab Agr Biosafety & Green Prod Upper Yangtze R, Chongqing 400715, Peoples R China
2.Guizhou Acad Agr Sci, Inst Hort, Guiyang 550006, Peoples R China
3.Chongqing Acad Forestry Sci, Chongqing Key Lab Forest Ecol Restorat & Utilizat, Chongqing 400036, Peoples R China
4.Southwest Univ, Coll Hort & Landscape Architecture, Chongqing 400715, Peoples R China
5.Sichuan Acad Agr Sci, Hort Res Inst, Garden & Flower Res Ctr, Chengdu 610000, Peoples R China
关键词：
MADS-box;
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 15 期
页码：
收录情况： SCI
摘要： Chimonanthus praecox, a traditional ornamental plant in China, is admired for its ability to bloom during the cold winter season and is recognized as an outstanding woody cut flower. MADS-box genes encode transcription factors essential for plant growth and development, with key functions in regulating flowering time and the formation of floral organs. In this study, 74 MADS-box genes (CpMADS1-CpMADS74) were identified and mapped across 11 chromosomes, with chromosome 1 harboring the highest number (13 genes) and chromosome 3 the fewest (3 genes). Physicochemical property analysis revealed that all CpMADS proteins are hydrophilic and predominantly nuclear-localized. Phylogenetic analysis classified these genes into Type I and Type II subfamilies, highlighting a clear divergence in domain structure. Eighty simple sequence repeat (SSR) loci were detected, with dinucleotide repeats being the most abundant, and the majority located in Type II MADS genes. From 23 C. praecox samples, 10 polymorphic SSR markers were successfully developed and PCR-validated, enabling a cluster analysis that grouped these cultivars into three distinct clusters. This study offers significant insights into the regulation of flowering, floral organ development, genetic linkage map construction, and the application of marker-assisted selection in C. praecox.
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