文献类型： 外文期刊

作者： Li, Zhihua ¹ ; Zhao, Chi ¹ ; Mao, Zhenyu ² ; Zhang, Fengju ¹ ; Dong, Ling ¹ ; Song, Chuan ² ; Chen, Yao ² ; Fu, Xin ² ; Ao, Zonghua ² ; Xiong, Yanfei ² ; Hui, Qin ² ; Song, Weizhi ⁴ ; Penttinen, Petri ⁵ ; Zhang, Suyi ² ;

作者机构： 1.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu 610066, Sichuan, Peoples R China

2.Luzhou Laojiao Co Ltd, Luzhou 646000, Peoples R China

3.Natl Engn Res Ctr Solid State Brewing, Luzhou 646000, Peoples R China

4.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Clear Water Bay, Hong Kong, Peoples R China

5.Sichuan Agr Univ, Coll Resources, Chengdu 611130, Peoples R China

6.Univ Helsinki, Fac Agr & Forestry, Helsinki 00014, Finland

关键词： Pit mud; Evolutionary divergence; Metagenome assembled genomes; Horizontal gene transfer; Carboxyl metabolites

期刊名称：ENVIRONMENTAL MICROBIOME （ 影响因子：5.4； 五年影响因子：6.4 ）

ISSN：

年卷期： 2025 年 20 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundPit mud (PM) hosts diverse microbial communities, which serve as a medium to impart flavor and quality to Baijiu and exhibit long-term tolerance to ethanol and acids, resulting in a unique ecosystem. However, the ecology and metabolic functions of PM remain poorly understood, as many taxa in PM represent largely novel lineages. In this study, we used a combination of metagenomic analysis and chemical derivatization LC-MS analysis to provide a comprehensive overview of microbial community structure, metabolic function, phylogeny, horizontal gene transfer, and the relationship with carboxyl compounds in spatiotemporal PM samples.ResultsOur findings revealed three distinct stages in the spatiotemporal changes of prokaryotic communities in PM: an initial phase dominated by Lactobacillus, a transitional phase, and a final state of equilibrium. Significant variations in alpha- and beta-diversity were observed across different spatial and temporal PM samples. We identified 178 medium- and high-quality non-redundant metagenome-assembled genomes (MAGs), and constructed their phylogenetic tree, depicting their roles in the carbon, nitrogen, and sulfur cycles. The Wood-Ljungdahl pathway and reverse TCA cycle were identified as the main carbon fixation mechanisms, with both hydrogenotrophic and aceticlastic methanogens playing a major role in methane production, and methylotrophic pathway observed in older PM. Furthermore, we identified relationships between prokaryotes and 29 carboxyl metabolites, including medium- and long-chain fatty acids. Horizontal gene transfer (HGT) was widespread in PM, particularly among clostridia, Bacteroidota, Bacilli, and Euryarchaeota, and was shown to play critical roles in fermentation dynamics, carbon fixation, methane production, and nitrogen and sulfur metabolism.ConclusionOur study provides new insights into the evolution and function of spatiotemporal PM, as well as its interactions with carboxyl metabolites. Lactobacillus dominated in new PM, while methanogens and clostridia were predominant in older or deeper PM layers. The three distinct stages of prokaryotic community development in PM and HGT played critical roles in metabolic function of spatiotemporal PM. Furthermore, this study highlights the importance of alpha-diversity, beta-diversity, methanogens, and Clostridium as useful indicators for assessing PM quality in the production of high-quality Baijiu.