文献类型： 外文期刊

作者： Feng, Xin ¹ ; Wang, Guangqiang ¹ ; Chen, Yunhui ¹ ; Xiong, Zhiqiang ¹ ; Xia, Yongjun ¹ ; Nan, Zhao ² ; Wenwen, Lu ³ ; Ling, Ni ⁵ ; Ying, Zhou ⁵ ; Ai, Lianzhong ¹ ;

作者机构： 1.Univ Shanghai Sci & Technol, Shanghai Engn Res Ctr Food Microbiol, Sch Hlth Sci & Engn, Shanghai, Peoples R China

2.Sichuan Acad Agr Sci, Inst Agroprod Proc Sci & Technol, Chengdu, Peoples R China

3.Wuxi Inst Specialized Nutr & Hlth Co Ltd, Wuxi, Peoples R China

4.Jiangnan Univ, Natl Engn Res Ctr Funct Food, State Key Lab Food Sci & Resources, Wuxi, Peoples R China

5.Univ Shanghai Sci & Technol, Dept Geriatr, Shidong Hosp, Shanghai, Peoples R China

6.Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Food Sci & Technol, Shanghai 200240, Peoples R China

关键词： Promoter; Bile salt; Oxygen; Lactiplantibacillus plantarum

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 68 卷

页码：

收录情况： SCI

摘要： In this study, transcriptome analysis was performed to identify the genes that confer oxygen tolerance and bile salt tolerance in Lactiplantibacillus plantarum AR113, a probiotic bacterium with potential applications in gastrointestinal environments. The plasmid pIB184 was selected as the vector for gene overexpression, with red fluorescent protein (RFP) used as the reporter gene to facilitate the identification and characterization of anaerobic promoters and bile salt-inducible promoters. This system was employed to screen for anaerobic promoters, and the activities of P2441,P1149, P2418 were found to be upregulated by 5.04-fold, 2.61-fold, and 3.03fold, respectively. P2528,P16090,Pshsp1,Pshsp2,Pshsp3 and Pgrosel exhibited increased activity under 0.1 % bile salt conditions, with fold changes of 1.75, 1.41, 1.99, 2.16, 2.10, and 1.59, respectively. Glycocholate and glycochenodeoxycholate were identified as the bile salts that most strongly induced the activity of these six bile salt-inducible promoters. The results of this study provide valuable insights into the regulatory mechanisms underlying bile salt and oxygen stress tolerance in L. plantarum AR113 and may be useful for the development of engineered lactic acid bacteria or functional strains capable of surviving in harsh gastrointestinal conditions.