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Molecular identification and development of an infectious cDNA clone of Trichosanthes kirilowii-infecting cucurbit mild mosaic virus

文献类型： 外文期刊

作者： Chen, Cheng 1 ; Du, Min 1 ; Wang, Yaqin 3 ; Zhou, Xueping 1 ; Yang, Xiuling 1 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing, Peoples R China

2.Sichuan Acad Agr Sci, Key Lab Integrated Pest Management Crops Southwest, Inst Plant Protect, Minist Agr, Chengdu, Peoples R China

3.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou, Peoples R China

关键词： Cucurbit mild mosaic virus; Fabavirus; Cucurbitaceae; Infectious clone; Mechanical transmission

期刊名称：VIROLOGY （ 影响因子：3.7； 五年影响因子：3.2 ）

ISSN： 0042-6822

年卷期： 2023 年 588 卷

页码：

收录情况： SCI

摘要： Trichosanthes kirilowii has been mainly grown for use in traditional Chinese medicine. In this study, cucurbit mild mosaic virus (CuMMV) belonging to the genus Fabavirus was identified from T. kirilowii plants. CuMMV possesses a segmented, bipartite linear single-stranded RNA genome composed of RNA1 and RNA2. Sequence analysis showed that each genomic segment shares the highest sequence similarity with those of CuMMV isolated from pumpkin. A full-length infectious cDNA clone of CuMMV was further constructed and was found to induce typical symptoms in T. kirilowii, Cucumis sativus, C. melo, Citrullus lanatus, and Cucurbita pepo. The sap inoculum derived from the infectious cDNA clone of CuMMV could be mechanically transmitted and reproduce similar symptoms in the tested plants. This is the first report on the construction of a biologically active, full-length infectious cDNA clone of CuMMV, which will provide a useful tool in understanding CuMMV-encoded proteins and plant-CuMMV interactions.

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