文献类型: 外文期刊
作者: Xia, Hui 1 ; Shen, Yanqiu 1 ; Hu, Rongping 2 ; Wang, Jin 1 ; Deng, Honghong 1 ; Lin, Lijin 1 ; Lv, Xiulan 1 ; Deng, Qunxian 1 ; Xu, Kunfu 1 ; Liang, Dong 1 ;
作者机构: 1.Sichuan Agr Univ, Coll Hort, Chengdu 611130, Peoples R China
2.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China
关键词: anthocyanin; coloration difference; DNA methylation; grape; MYBA1; UFGT
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.895; 五年影响因子:6.048 )
ISSN: 0021-8561
年卷期: 2021 年 69 卷 51 期
页码:
收录情况: SCI
摘要: The "Manicure Finger" grape is notable for its fingerlike berries with a bright red top and yellow base; however, the mechanism underlying this color difference remains unknown. This study showed that the anthocyanin concentration and the expression levels of anthocyanin-related genes in the top skin were notably higher than those in the basal skin. The expression levels of DFR, UFGT, and GST were significantly correlated with the anthocyanin content. The promoters of the two VvUFGT alleles can be activated by VvMYBA1, which was verified by the yeast one-hybrid assay, the dual-luciferase reporter gene assay, and the electrophoretic mobility shift assay. Moreover, the methylation level of the VvMYBA1 promoter (-1488 to -1083 bp) in the top skin was significantly lower than that in the basal skin and was positively correlated with the anthocyanin content. Our data suggest that methylation levels of the VvMYBA1 promoter play a crucial role in regulating grape skin coloration.
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