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Molecular Scree ning of Blast Resistance Genes in Rice Germplasms Resistant to Magnaporthe oryzae

文献类型: 外文期刊

作者: Liang Yan 1 ; Yan Bai-yuan 2 ; Peng Yun-liang 3 ; Ji Zhi-juan 1 ; Zeng Yu-xiang 1 ; Wu Han-lin 1 ; Yang Chang-deng 1 ;

作者机构: 1.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 310006, Zhejiang, Peoples R China

2.Seed Management Stn Jiande, Jiande 311600, Peoples R China

3.Sichuan Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crops Southwes, Minist Agr, Chengdu 610066, Peoples R China

关键词: rice;blast resistance gene;field evaluation;marker-assisted selection

期刊名称:RICE SCIENCE ( 影响因子:3.333; 五年影响因子:4.226 )

ISSN: 1672-6308

年卷期: 2017 年 24 卷 1 期

页码:

收录情况: SCI

摘要: Molecular screening of major rice blast resistance genes was determined with molecular markers, which showed close-set linkage to 11 major rice blast resistance genes (Pi-d2, Pi-z, Piz-t, Pi-9, Pi-36, Pi-37, Pi5, Pi-b, Pik-p, Pik-h and Pi-ta(2)), in a collection of 32 accessions resistant to Magnaporthe oryzae. Out of the 32 accessions, the Pi-d2 and Pi-z appeared to be omnipresent and gave positive express. As the second dominant, Pi-b and Piz-t gene frequencies were 96.9% and 87.5%. And Pik-h and Pik-p gene frequencies were 43.8% and 28.1%, respectively. The molecular marker linkage to Pi-ta(2) produced positive bands in eleven accessions, while the molecular marker linkage to Pi-36 and Pi-37 in only three and four accessions, respectively. The natural field evaluation analysis showed that 30 of the 32 accessions were resistant, one was moderately resistant and one was susceptible. Infection types were negatively correlated with the genotype scores of Pi-9, Pi5, Pi-b, Pi-ta(2) and Pik-p, although the correlation coefficients were very little. These results are useful in identification and incorporation of functional resistance genes from these germplasms into elite cultivars through marker-assisted selection for improved blast resistance in China and worldwide.

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