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Engineering sulfur storage in maize seed proteins without apparent yield loss

文献类型： 外文期刊

作者： Planta, Jose 1 ; Xiang, Xiaoli 2 ; Leustek, Thomas 2 ; Messing, Joachim 1 ;

作者机构： 1.Rutgers State Univ, Waksman Inst Microbiol, Piscataway, NJ 08854 USA

2.Rutgers State Univ, Dept Plant Biol, New Brunswick, NJ 08901 USA

3.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol, Chengdu 610061, Sichuan, Peoples R China

关键词： APS reductase;sulfur assimilation;sulfur-rich zeins;10-kDad-zein

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：11.205； 五年影响因子：12.291 ）

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收录情况： SCI

摘要： Sulfur assimilation may limit the pool of methionine and cysteine available for incorporation into zeins, the major seed storage proteins in maize. This hypothesis was tested by producing trans-genic maize with deregulated sulfate reduction capacity achieved through leaf-specific expression of the Escherichia coli enzyme 3'-phosphoadenosine-5'-phosphosulfate reductase (EcPAPR) that resulted in higher methionine accumulation in seeds. The transgenic kernels have higher expression of the methionine-rich 10-kDa delta-zein and total protein sulfur without reduction of other zeins. This overall increase in the expression of the S-rich zeins describes a facet of regulation of these proteins under enhanced sulfur assimilation. Transgenic line PE5 accumulates 57.6% more kernel methionine than the high-methionine inbred line B101. In feeding trials with chicks, PE5 maize promotes significant weight gain compared with nontrans-genic kernels. Therefore, increased source strength can improve the nutritional value of maize without apparent yield loss and may significantly reduce the cost of feed supplementation.

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