文献类型： 外文期刊
作者： Jia, Dinghong 2 ; Wang, Bo 2 ; Li, Xiaolin 2 ; Peng, Weihong 2 ; Zhou, Jie 2 ; Tan, Hao 2 ; Tang, Jie 2 ; Huang, Zhongqia 1 ;
作者机构： 1.Sichuan Univ, Key Lab Biol Resource & Ecol Environm, Minist Educ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Sichuan, Peoples R China
3.Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Sichuan, Peo
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）
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收录情况： SCI
摘要： Auricularia polytricha is one of the most widely cultivated edible mushrooms in China. Many advances have been made to A. polytricha, but there is still no proteomic information of this species. Our current understanding was based upon the translated information of its transcriptome or other relative species. This study presented the proteomic information of fruiting-body proteins by shotgun liquid chromatography and tandem mass spectrometry (LC-MS/MS), which identified 15,508 peptides corresponding to 1850 high-confidence proteins. Of these, 1383 were annotated across the GO subcategories with 829 (44.81%) involved in biological process, 908 (49.08%) in molecular function, and 406 (21.95%) in cellular components. Among these high-confidence proteins, 132 proteins were annotated as carbohydrate-active enzymes, of which 51 were secreted enzymes. Moreover, a number of commercially important enzymes were detected, functioning as auxiliary activity (AA) family 5 glyoxal oxidase, AA5 galactose oxidase, glycoside hydrolase (GH) family 20 hexosaminidase, and GH47 alpha-mannosidase. To the best of our knowledge, this is the first study to characterize A. polytricha proteome, and also fills the gap of our knowledge on the under-developed mushroom species.
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