文献类型： 外文期刊

作者： Zhao, Haoyu ¹ ; Tao, Ke ² ; Zhu, Jianyi ¹ ; Liu, Shengnan ¹ ; Gao, Han ¹ ; Zhou, Xiaogang ¹ ;

作者机构： 1.Sichuan Acad Agr Sci, Key Lab Integrated Pest Management Crops Southwes, Minist Agr, Inst Plant Protect, Chengdu 610066, Peoples R China

2.Sichuan Univ, Coll Life Sci, Minist Educ, Key Lab Bioresource & Ecoenvironm, Chengdu 610064, Peoples R China

关键词： bioremediation;degradation;glyphosate;metabolite;Pseudomonas

期刊名称：JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY （ 影响因子：1.452； 五年影响因子：1.479 ）

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收录情况： SCI

摘要： Bacterial strains capable of utilizing glyphosate as the sole carbon source were isolated from contaminated soil by the enrichment culture method and identified based on partial 16S rRNA gene sequence analysis. Pseudomonas spp. strains GA07, GA09 and GC04 demonstrated the best degradation capabilities towards glyphosate and were used for the laboratory experiments of glyphosate bioremediation. Inoculating glyphosate-treated soil samples with these three strains resulted in a 2-3 times higher rate of glyphosate removal than that in non-inoculated soil. The degradation kinetics was found to follow a first-order model with regression values greater than 0.96. Cell numbers of the introduced bacteria decreased from 4.4 x 10(6) CFU/g to 3.4-6.7 x 10(5) CFU/g dry soil within 18 days of inoculation. Due to the intense degradation of glyphosate, the total dehydrogenase activity of the soil microbial community increased by 21.2-25.6%. Analysis of glyphosate degradation products in cell-free extracts showed that glyphosate breakdown in strain GA09 was catalyzed both by C-P lyase and glyphosate oxidoreductase. Strains GA07 and GC04 degraded glyphosate only via glyphosate oxidoreductase, but no further metabolite was detected. These results highlight the potential of the isolated bacteria to be used in the bioremediation of GP-contaminated soils.