文献类型： 外文期刊

作者： Tao, L. ² ; Wang, Y. Q. ¹ ; Pan, C. P. ¹ ; Yang, Z. W. ¹ ; Deng, Q. X. ¹ ; Liu, J. ¹ ;

作者机构： 1.Sichuan Agr Univ, Coll Hort, Yaan 625014, Sichuan, Peoples R China

2.Sichuan Acad Agr Sci, Inst Hort, Chengdu 610066, Sichuan, Peoples R China

关键词： culture media;Eriobotrya japonica;genetic transformation;growth regulator;parthenocarpy gene

期刊名称：IV INTERNATIONAL SYMPOSIUM ON LOQUAT

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收录情况： SCI

摘要： The parthenocarpic gene iaaM was transferred into anther-derived embryos, using Agrobacterium-mediated transformation. The results showed that 2-3-day pre-culture, 15-min infection and 3-day co-culture with 10 mg L~(-1) AS in the co-culture medium ledto an increase in transformation efficiency. Cefotaxime at 250 mg L~(-1) or Ampicillin at 750 mg L~(-1) were optimal. Sixteen transgenic lines were obtained. The iaaM gene-specific amplification results showed that 9 strains were amplified at the expected target bands, and the PCR positive rate was 56.3%. The PCR-Southern analysis indicated that 8 strains showed hybridization signal, which further proved that the iaaM gene was integrated into the genome of the embryos, and the transformation rate was about 0.5%.