Rhizosphere Inhibition of Cucumber Fusarium Wilt by Different Surfactin-excreting Strains of Bacillus subtilis
文献类型： 外文期刊
作者： Jia, Ke 1 ; Gao, Yu-Han 1 ; Huang, Xiao-Qin 2 ; Guo, Rong-Jun 1 ; Li, Shi-Dong 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Minist Agr, Key Lab Pest Management Crops, Beijing 100081, Peoples R China
2.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China
关键词： colonization;Fusarium oxysporum f. sp. cuc-umerinum;real-time PCR;surfactin
期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）
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收录情况： SCI
摘要： Bacillus subtilis B006 strain effectively suppresses the cucumber fusarium wilt caused by Fusarium oxyspo-rum f. sp. cucumerinum (Foe). The population dynamics of Foe, strain B006 and its surfactin over-producing mutant B841 and surfactin-deflcient mutant B1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopep-tides excreted by these strains in suppressing Foe. Results showed that B. subtilis strain B006 effectively suppressed the disease in natural soil by 42.9%, five weeks after transplanting, whereas B841 and B1020 suppressed the disease by only 22.6% and 7.1%, respectively. Quantitative PCR assays showed that effective colonization of strain B006 in the rhizosphere suppressed Foe propagation by more than 10 times both in nursery substrate and in field-infected soil. Reduction of Foe population at the cucumber stems in a range of 0.96 logl0 ng/g to 2.39 log,,, ng/g was attained at the third and the fifth weeks of B006 treatment in nurserysubstrate. In Field-infected soil, all three treatments with B. subtilis suppressed Foe infection, indicated by the reduction of Foe population at a range of 2.91 log10 ng/g to 3.36 log,,, ng/g at the stem base, one week after transplanting. This study reveals that the suppression of fusarium wilt disease is affected by the effective colonization of the surfactin-producing B. subtilis strain in the rhizosphere. These results improved our understanding of the biocontrol mechanism of the B. subtilis strain B006 in the natural soil and facilitate its application asbiocontrol agent in the field.
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