文献类型： 外文期刊
作者： Wu, Liuji 2 ; Hu, Xiuli 1 ; Tang, Haitao 4 ; Han, Zanping 5 ; Chen, Yanhui 1 ;
作者机构： 1.Henan Agr Univ, Zhengzhou 450002, Peoples R China
2.Synerget Innovat Ctr Henan Grain Crops, Zhengzhou 450002, Peoples R China
3.Key Lab Physiol Ecol & Genet Improvement Food Cro, Zhengzhou 450002, Peoples R China
4.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China
5.Henan Univ Sci & Technol, Coll Agron, Luoyang 471003, Peoples R China
关键词： Western blotting;Systematic errors;Reference proteins;Normalization;Accuracy
期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）
ISSN：
年卷期：
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收录情况： SCI
摘要： Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. However, the systematic errors in the application of western blotting analysis are frequently to be found, which may compromise the interpretation of results. To make a valid application of western blotting, it is essential to begin with three independent biological replicates. Subsequently, a more reliable normalization method is in urgent need for western blotting analysis and using reference proteins is the currently preferred method of normalization. Additionally, identification of valid reference proteins is crucial for western blotting analysis and it should be examined carefully in relation to the cell or tissue types when using housekeeping proteins as internal standards.
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