文献类型： 外文期刊
作者： Dai, Shoufen 1 ; Pu, Zongjun 2 ; Liu, Dengcai 1 ; Wei, Yuming 1 ; Zheng, Youliang 1 ; Hu, Xinkun 1 ; Yan, Zehong 1 ;
作者机构： 1.Sichuan Agr Univ, Triticeae Res Inst, Chengdu 611130, Sichuan, Peoples R China
2.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China
关键词： Eremopyrum distans;Eremopyrum triticeum;Evolution;F genome;Gene sequencing;Glu-1;Xe genome
期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）
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收录情况： SCI
摘要： Three HMW-GS and the respective ORFs from diploid species Eremopyrum distans and Eremopyrum triticeum were characterized. Compared to homologous proteins, they showed novel modifications in all domains. In the N-terminals, the y subunit from Er. triticeum (Xey) had 98 aa residues. A short G/IIFWGTS peptide deletion was responsible for the reduced number of aa residues. The end peptide in the y subunit from Er. distans (Fy) was IPTLLR. This unique structure was involved in a replacement between x types with IPA/TLLK/R and y types with R/TSSQTVQ. Both y subunits share the same short peptide LAAQLPAMCRL as x types in the C-terminals. Phylogenic relationships among orthologous genes from Triticeae species revealed that Fy and Xey were neither purely x type nor purely y type based on the N and C terminal residues. Divergence times indicated that Glu-Xe1 and Glu-F1 were separated from each other and that Glu-Xe1 separated from orthologous loci of wild wheat relatives earlier than Glu-F1. Based on the divergence times among Glu-F1, Glu-Xe1, Glu-O1, Glu-St1, and Glu-Ta1, it is possible that genome F separation from O, St, and Ta in species of Henrardia persica, Pseudoroegneria stipifolia, and Taeniatherum crinitum was more recent than the separation of F and Xe. ? 2013 Elsevier B.V.
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