Isolation of an anti-tumor polysaccharide from Auricularia polytricha (Jew's ear) and its effects on macrophage activation
文献类型： 外文期刊
作者： Yu, Mengyao 3 ; Xu, Xiaoyan 3 ; Qing, Yuan 1 ; Luo, Xia 3 ; Yang, Zhirong 1 ; Zheng, Linyong 1 ;
作者机构： 1.Sichuan Univ, Coll Life Sci, Chengdu 610064, Peoples R China
2.Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Peoples R China
3.Sichuan Inst Chinese Mat Med, Chengdu 610041, Peoples R China
关键词： Auricularia polytricha;Polysaccharides;Anti-tumor;Macrophage activation;Cytokine;NO
期刊名称：EUROPEAN FOOD RESEARCH AND TECHNOLOGY （ 影响因子：2.998； 五年影响因子：3.005 ）
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收录情况： SCI
摘要： To investigate the active compound and its mechanism of the anti-tumor effects of Auricularia polytri-cha, the polysaccharides were isolated and its effects on macrophages were tested. By alcohol-precipitation, DEAE-cellulose ion exchange, gel permeation chromatography and in vivo anti-tumor test on the sarcoma 180 tumor-bearing mice, a fraction of polysaccharides (namely APPIIA) with significant anti-tumor effect was isolated. APPIIA can enhance the neutral red phagocytosis of macrophages. It can also stimulate the nitric oxide (NO) release and cytokines secretion of macrophage in a dose-dependent manner. By quantitative RT-PCR method, it can be found that APPIIA can augment the mRNA transcription of inducible nitric oxide synthase (iNOS), tumor necrosis factor (TNF)-alpha, interleukin (IL)-l beta and IL-6. The results of western blotting showed that it can also increased the protein expression of iNOS. These results suggest that APPIIA is one of effective anti-tumor agents and its anti-tumor effect may exhibit via the macrophage activation.
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