Molecular Diversity of Auricularia polytricha Revealed by Inter-Simple Sequence Repeat and Sequence-Related Amplified Polymorphism Markers
文献类型： 外文期刊
作者： Yu, Mengyao 2 ; Ma, Bo 1 ; Luo, Xia 2 ; Zheng, Linyong 3 ; Xu, Xiaoyan 2 ; Yang, Zhirong 1 ;
作者机构： 1.Sichuan Univ, Key Lab Biol Resource & Ecol Environm, Minist Educ, Coll Life Sci, Chengdu 610064, Sichuan, Peoples R China
2.Sichuan Inst Chinese Mat Medi, Cellular & Mol Lab Chinese Mat Medi, Chengdu, Sichuan, Peoples R China
3.Sichuan Acad Agr Sci, Soil & Fertil Inst, Chengdu, Sichuan, Peoples R China
期刊名称：CURRENT MICROBIOLOGY （ 影响因子：2.188； 五年影响因子：2.197 ）
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收录情况： SCI
摘要： Due to unsatisfying attempts to fingerprint Auricularia polytricha, two different molecular maker systems--Inter-Simple Sequence Repeats (ISSR) and Sequence-related amplified polymorphism (SRAP)--were established and tested to quantify molecular diversity among 19 strains of this fungus. A total of 202 (99.0%) and 459 (95.9%) polymorphic bands were detected by 13 ISSR primers and 14 SRAP primer combinations, respectively. By parsimony method, a phylogenetic tree was constructed based on each analysis; the two trees show that 19 A. polytricha strains were distributed into five or four groups. These results demonstrated that both methods were suitable for discriminating among strains of A. polytricha, and the novel SRAP markers are more efficient and preferable. The result also indicated the high level of genetic diversity of A. polytricha and their relationship between each other. These findings would benefit future research in A. polytricha, especially in breeding and medicine development. It also gives a useful method for fingerprinting of other fungi.
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