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MicroRNA bmo-miR-31-5p inhibits apoptosis and promotes BmNPV proliferation by targeting the CYP9e2 gene of Bombyx mori

文献类型： 外文期刊

作者： Yang, Wen-Yu 2 ; Liu, Zhen-Ye 2 ; Zhu, Yan 2 ; Xiao, Yu 2 ; Xiao, Wen-Fu 3 ; Tang, Liang 4 ; Dong, Zhan-Qi 2 ; Pan, Min-Hui 2 ; Lu, Cheng 1 ; Chen, Peng 1 ;

作者机构： 1.Southwest Univ, State Key Lab Resource Insects, 2 Tiansheng Rd, Chongqing, Peoples R China

2.Southwest Univ, State Key Lab Resource Insects, Key Lab Sericultural Biol & Genet Breeding, Minist Agr & Rural Affairs, Beibei, Peoples R China

3.Sichuan Acad Agr Sci, Sericultural Res Inst, Nanchong, Peoples R China

4.Sericulture Technol Promot Stn Guangxi, Nanning, Peoples R China

关键词： silkworm; apoptosis; BmNPV; miRNA; miR-31-5p; BmCYP9e2

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.1； 五年影响因子：4.4 ）

ISSN： 1526-498X

年卷期： 2024 年

页码：

收录情况： SCI

摘要： BACKGROUND: Bombyx mori nuclear polyhedrosis virus (BmNPV), as a typical baculovirus, is the primary pathogen that infects the silkworm B. mori, a lepidopteran species. Owing to the high biological safety of BmNPV in infecting insects, it is commonly utilized as a biological insecticide for pest control. Apoptosis is important in the interaction between the host and pathogenic microorganisms. MicroRNAs (miRNAs) influence immune responses and promote stability of the immune system via apoptosis. Therefore, the study of apoptosis-related miRNA in silkworms during virus infection can not only provide support for standardizing the prevention and control of diseases and insect pests, but also reduce the economic losses to sericulture caused by the misuse of biological pesticides. RESULTS: Through transcriptome sequencing, we identified a miRNA, miR-31-5p, and demonstrated that it can inhibit apoptosis in silkworm cells and promote the proliferation of BmNPV in BmE-SWU1 cells. We identified a target gene of miR-31-5p, B. mori cytochrome P450 9e2 (BmCYP9e2), and demonstrated that it can promote apoptosis in silkworm cells and inhibit the proliferation of BmNPV. Moreover, we constructed transgenic silkworm strains with miR-31-5p knockout and confirmed that they can inhibit the proliferation of BmNPV. CONCLUSION: These data indicate that miR-31-5p may exert functions of inhibiting apoptosis and promoting virus proliferation by regulating BmCYP9e2. The findings demonstrate how miRNAs influence host cell apoptosis and how they are involved in the host immune system response to viruses, providing important insights into the applications of biological insecticides for pest control. (c) 2024 Society of Chemical Industry.

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