Molecular cloning, expression and appetite regulation function of adiponectin in Siberian sturgeon (Acipenser baerii)
文献类型： 外文期刊
作者： Tang, Ni 1 ; Li, Ya 1 ; Li, Yingzi 1 ; Liu, Yanling 1 ; Zhang, Shupeng 1 ; Xu, Shaoqi 1 ; Wang, Mei 1 ; Wang, Bin 2 ; Chen, Hu 1 ; Zhang, Xin 1 ; Zhou, Bo 2 ; Chen, Defang 1 ; Li, Zhiqiong 1 ;
作者机构： 1.Sichuan Agr Univ, Coll Anim Sci & Technol, Dept Aquaculture, 211 Huimin Rd, Chengdu 610000, Peoples R China
2.Sichuan Acad Agr Sci, Fisheries Inst, 156 Gaozhuang Bridge Community, Yibin, Sichuan, Peoples R China
3.Sichuan Agr Univ, Coll Anim Sci & Technol, Dept Aquaculture, Chengdu, Peoples R China
关键词： Adiponectin; Appetite; Food intake
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.025； 五年影响因子：7.626 ）
ISSN： 0141-8130
年卷期： 2022 年 214 卷
页码：
收录情况： SCI
摘要： Adiponectin (AdipoQ) as an adipocytokine has the potential to regulate feeding behavior, but the information about adipoq in fish is limited. In this study, Siberian sturgeon adiponectin (Ssadipoq) gene was cloned encoding 264 amino acids. The amino acid identity of SsAdipoQ was low compared with that of mammals, birds, amphibians and teleost fishes. The expression of Ssadipoq in the hypothalamus was significantly decreased at 1 h and 3 h post feeding, and increased after 15-day fasting. The mature domain of AdipoQ (fAd) was inserted into expression vector pET32a and successfully expressed in Escherichia coli BL21 (DE3) after stimulated by isopropyl beta-d-thiogalactoside. Food intake at 1 h and 3 h post treatment with SsfAd protein decreased significantly (P < 0.05). The mRNA expression of pyy and cck in the valvula intestine was promoted and hypothalamic npy, agrp and pomc mRNA expression were inhibited after treatment with SsfAd protein. Furthermore, hypothalamic ampk subunits expression was associated with peripheral SsfAd treatment. In summary, present study indicate that SsfAd plays an important role in the regulation of food intake and appetite signals in Siberian sturgeon, which provides a basis for further study application of prokaryotic AdipoQ in feeding behavior regulation.
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