文献类型： 外文期刊

作者： Sun, Shu Xia ² ; Gao, Fang Yuan ¹ ; Lu, Xian Jun ¹ ; Wu, Xian Jun ² ; Wang, Xu Dong ² ; Ren, Guang Jun ¹ ; Luo, Hong ³ ;

作者机构： 1.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China

2.Sichuan Agr Univ, Rice Res Inst, Chengdu, Peoples R China

3.Clemson Univ, Dept Biochem & Genet, Clemson, SC USA

关键词： Aroma;Gene fine mapping;Genetic analysis;Rice (Oryza sativa L.)

期刊名称：GENETICS AND MOLECULAR BIOLOGY （ 影响因子：1.771； 五年影响因子：2.584 ）

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收录情况： SCI

摘要： We investigated inheritance and carried out gene fine mapping of aroma in crosses between the aromatic elite hybrid rice Oryza sativa indica variety Chuanxiang-29B (Ch-29B) and the non-aromatic rice O. sativa indica variety R2 and O. sativa japonica Lemont (Le). The F grains and leaves were non-aromatic while the F_2 non-aroma to aroma segregation pattern was 3:1. The F_3 segregation ratio was consistent with the expected 1:2:1 for a single recessive aroma gene in Ch-29B. Linkage analysis between simple sequenbe repeat (SSR) markers and the aroma locus for the aromatic F_2 plants mapped the Ch-29B aroma gene to a chromosome 8 region flanked by SSR markers RM23120 at 0.52 cM and RM3459 at 1.23 cM, a replicate F_2 population confirming these results. Three bacterial artificial chromosome (BAC) clones cover chromosome 8 markers RM23120 and RM3459. Our molecular mapping data from the two populations indicated that the aroma locus occurs in a 142.85 kb interval on BAC clones AP005301 or AP005537, implying that it might be the same gene reported by Bradbury et al (2005a; Plant Biotec J. 3:363-370). The flanking markers Aro7, RM23120 and RM3459 identified by us could greatly accelerate the efficiency and precision of aromatic rice breeding programs.