文献类型： 外文期刊
作者： Wu, Wenqiong 1 ; Qiu, Junqiang 1 ; Lin, Yue 1 ; Li, Xike 1 ; Li, Wenjuan 1 ; Duan, Yuanliang 3 ; Fu, Yuanshuai 1 ;
作者机构： 1.Shanghai Ocean Univ, Minist Agr & Rural Affairs, Key Lab Freshwater Aquat Genet Resources, Shanghai, Peoples R China
2.Shanghai Ocean Univ, Minist Educ, Key Lab Explorat & Utilizat Aquat Genet Resources, Shanghai, Peoples R China
3.Sichuan Acad Agr Sci, Fisheries Res Inst, Chengdu, Peoples R China
关键词： Triplophysa tenuis; SSR; RAD-seq; genetic diversity; population structure
期刊名称：FRONTIERS IN MOLECULAR BIOSCIENCES （ 影响因子：3.9； 五年影响因子：4.4 ）
ISSN：
年卷期： 2024 年 11 卷
页码：
收录情况： SCI
摘要： To investigate the genetic diversity of Triplophysa tenuis in the Shule River Basin of Gansu province, three populations were sequenced via RAD-seq technology. Twenty-nine microsatellite (SSR) markers with polymorphisms were finally screened to access the genetic diversity among the populations, of which 15 had high polymorphisms. The quantity of the alleles detected in the three populations of T. tenuis varied from 2 to 24. The locus with the most alleles was SSRC1, which had 24 alleles. Among the 29 SSRs, the range of effective allele number, observed heterozygosity, expected heterozygosity, and polymorphic information content were 1.246-16.615, 0.222-1, 0.198-0.940, and 0.178-0.937, respectively. Most of the identified loci were in the Hardy-Weinberg equilibrium. Analysis of the population structure revealed that the Yumen and Changma populations shared the same origin, while the Qiaowan population was different from them. The developed SSR markers discovered in this study will contribute to the conservation research on T. tenuis and the conservation of the fishery resources of the Shule River, providing scientific guidance for the development and utilization of T. tenuis resources and environmental protection.
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