您好，欢迎访问四川省农业科学院 机构知识库！

Wheat glutamine synthetase TaGSr-4B is a candidate gene for a QTL of thousand grain weight on chromosome 4B

文献类型： 外文期刊

作者： Yang, Fan 1 ; Zhang, Jingjuan 1 ; Zhao, Yun 1 ; Liu, Qier 1 ; Islam, Shahidul 1 ; Yang, Wuyun 2 ; Ma, Wujun 1 ;

作者机构： 1.Murdoch Univ, Coll Sci Hlth Engn & Educ, Australian China Joint Ctr Wheat Improvement, Food Futures Inst, 90 South St, Murdoch, WA 6150, Australia

2.Sichuan Acad Agr Sci, Crop Res Inst, 4 Shizishan Rd, Chengdu 610066, Peoples R China

3.Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.574； 五年影响因子：5.662 ）

ISSN： 0040-5752

年卷期： 2022 年 135 卷 7 期

页码：

收录情况： SCI

摘要： Key message Glutamine synthetase TaGSr-4B is a candidate gene for a QTL of thousand grain weight on 4B, and the gene marker is ready for wheat breeding. A QTL for thousand grain weight (TGW) in wheat was previously mapped on chromosome 4B in a DH population of Westonia x Kauz. For identifying the candidate genes of the QTL, wheat 90 K SNP array was used to saturate the existing linkage map, and four field trials plus one glasshouse experiment over five locations were conducted to refine the QTL. Three nitrogen levels were applied to two of those field trials, resulting in a TGW phenotype data set from nine environments. A robust TGW QTL cluster including 773 genes was detected in six environments with the highest LOD value of 13.4. Based on differentiate gene expression within the QTL cluster in an RNAseq data of Westonia and Kauz during grain filling, a glutamine synthesis gene (GS: TaGSr-4B) was selected as a potential candidate gene for the QTL. A SNP on the promoter region between Westonia and Kauz was used to develop a cleaved amplified polymorphic marker for TaGSr-4B gene mapping and QTL reanalysing. As results, TGW QTL appeared in seven environments, and in four out of seven environments, the TGW QTL were localized on the TaGSr-4B locus and showed significant contributions to the phenotype. Based on the marker, two allele groups of Westonia and Kauz formed showed significant differences on TGW in eight environments. In agreement with the roles of GS genes on nitrogen and carbon remobilizations, TaGSr-4B is likely the candidate gene of the TGW QTL on 4B and the TaGSr-4B gene marker is ready for wheat breeding.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：四川省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台