文献类型： 外文期刊
作者： Liang, Ying 1 ; Li, Chun 1 ; Mangauthia, Satendra Kumar 2 ; Biswal, Akshaya Kumar 3 ;
作者机构： 1.Sichuan Acad Agr Sci, Vegetable Germplasm Innovat & Variety Improvement, Hort Res Inst, Chengdu 610066, Sichuan, Peoples R China
2.Indian Inst Rice Res, Hyderabad 500030, India
3.Univ Georgia, Dept Hort, Tifton, GA 31793 USA
关键词： CRISPR/Cas9; Genome editing; Accelerated breeding; Rice; Wheat; Maize Disease resistance
期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：1.9； 五年影响因子：1.6 ）
ISSN： 0971-7811
年卷期： 2023 年 32 卷 4 期
页码：
收录情况： SCI
摘要： Identifying and characterizing genes that control important agronomic traits and finding ways to alter them are vital for crop improvement. Map-based cloning, heterologous gene expression, RNAi mediated gene silencing, T-DNA insertional mutation and TILLING technologies have enabled cloning, characterization, and deployment of a few important genes. Revolution in sequencing technologies and bioinformatics has facilitated us to quickly predict and annotate plethora of genes and regulatory elements with improved precision and spatio-temporal expression of genes in almost all cereal crops. However, their functional validation forms a bottleneck in exploiting useful genetic elements for crop improvement. The CRISPR/Cas9 mediated gene editing has become the tool of choice for precisely introducing targeted modifications in the genome to knock out/in a gene, introducing specific mutation in sequence or modulating its expression in diverse crop species. This can help to rapidly characterize plenty of genes in terms of understanding the function of individual gene/ gene family, involvement in a particular biochemical pathway or interaction of the crop plant with external stimuli at a reasonable cost. In this review, we discuss the power of gene editing for rapid functional characterization of genetic elements as a fundamental requirement to harness the power of precision genetic technologies for increasing crop yield, progress made so far, opportunities and challenges.
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