文献类型： 外文期刊

作者： Wang Zhen-zhong ¹ ; Xie Jing-zhong ¹ ; Guo Li ² ; Zhang De-yun ² ; Li Gen-qiao ³ ; Fang Ti-lin ³ ; Chen Yong-xing ¹ ; Li ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China

2.China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China

3.Oklahoma State Univ, Dept Plant & Soil Sci, Stillwater, OK 74078 USA

4.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China

5.China Natl Seed Grp Co Ltd, Beijing 100045, Peoples R China

6.Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

7.Univ Haifa, Inst Evolut, IL-31905 Haifa, Israel

关键词： Triticum dicoccoides; Puccinia striiformis f. sp tritici; SNP; comparative genomics

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2018 年 17 卷 6 期

页码：

收录情况： SCI

摘要： Wheat stripe rust, caused by Puccinia striiiformis f. sp. tritici (Pst), is a devastating disease that can cause severe yield losses. Identification and utilization of stripe rust resistance genes are essential for effective breeding against the disease. Wild emmer accession TZ-2, originally collected from Mount Hermon, Israel, confers near-immunity resistance against several prevailing Pst races in China. A set of 200 F-6.7 recombinant inbred lines (RILs) derived from a cross between susceptible durum wheat cultivar Langdon and TZ-2 was used for stripe rust evaluation. Genetic analysis indicated that the stripe rust resistance of TZ-2 to Pst race CYR34 was controlled by a single dominant gene, temporarily designated YrTZ2. Through bulked segregant analysis (BSA) with SSR markers, YrTZ2 was located on chromosome arm 1BS flanked by Xwmc230 and Xgwm413 with genetic distance of 0.8 cM (distal) and 0.3 cM (proximal), respectively. By applying wheat 90K iSelect SNP genotyping assay, 11 polymorphic loci (consisting of 250 SNP markers) closely linked to YrTZ2 were identified. YrTZ2 was further delimited into a 0.8-cM genetic interval between SNP marker 1WB19368 and SSR marker Xgwm413, and co-segregated with SNP marker IWB28744 (co-segregated with 28 SNP). Comparative genomics analyses revealed high level of collinearity between the Yr TZ2 genomic region and the orthologous region of Aegilops tauschii 1DS. The genomic region between loci IW1319368 and IWB31649 harboring YrTZ2 is orthologous to a 24.5-Mb genomic region between AT1D0112 and AT1D0150, spanning 15 contigs on chromosome 1DS. The genetic and comparative maps of YrTZ2 provide a framework for map-based cloning and marker-assisted selection of YrTZ2.