Engineering High-Amylose and High-Dietary-Fibre Barley Grains Through Multiplex Genome Editing of Four Starch-Synthetic Genes
文献类型： 外文期刊
作者： Yang, Qiang 1 ; Ral, Jean-Philippe 3 ; Jiang, Qiantao 2 ; Li, Zhongyi 3 ;
作者机构： 1.Sichuan Acad Agr Sci, Inst Qual Stand & Testing Technol Res, Chengdu 610066, Peoples R China
2.Sichuan Agr Univ, Triticeae Res Inst, State Key Lab Crop Gene Explorat & Utilisat Southw, Chengdu Campus, Chengdu 611130, Peoples R China
3.CSIRO, Agr & Food, Canberra, ACT 2601, Australia
关键词：
期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）
ISSN：
年卷期： 2025 年 14 卷 13 期
页码：
收录情况： SCI
摘要： Barley, rich in beneficial ingredients, has been recognised as a healthy food and is widely used in the production of healthy foods for humans. The current study identified a new barley mutant with the SSIIa, SSIIIa, SBEIIa, and SBEIIb genes inactivated in the genome-edited offspring of targeted mutagenesis of starch synthetic genes using multiplex genome editing. The grain compositions and starch properties of the ssIIa/ssIIIa/sbeIIa/sbeIIb mutant were analysed and compared with the corresponding parameters of ssIIa, ssIIIa, sbeIIa/sbeIIb, ssIIa/sbeIIa/sbeIIb, and non-genome-edited lines (NE), respectively. ssIIa/ssIIIa/sbeIIa/sbeIIb exhibited the highest contents of beta-glucan and amylose content among all mutants and NE, but not the most prominent in resistant starch, fructan, and fibre contents. The loss of SSIIa, SSIIIa, SBEIIa, and SBEIIb genes also resulted in significant changes in starch properties. This study enriched the genotypes of healthy barley and provided a theoretical basis for improving barley quality.
- 相关文献
作者其他论文 更多>>
-
Fine-mapping and candidate gene identification for QPtn.sau-4B showing potential in increasing productive tiller number and yield in wheat
作者：Liu, Jiajun;Wang, Tongzhu;Lan, Yuxin;Zhang, Zhiyuan;Wu, Lei;Hu, Xinrong;Yin, Li;Liu, Yanlin;Tang, Huaping;Jiang, Qiantao;Xu, Qiang;Chen, Guoyue;Qi, Pengfei;Jiang, Yunfeng;Wei, Yuming;Zheng, Youliang;Ma, Jian;Liu, Jiajun;You, Jianing;Liu, Chunji
关键词：Wheat; Productive tiller number; Grain yield; Candidate genes
-
Identification, characterization, and associations with agronomic traits and early vigor of mature wheat embryo size loci
作者：Wu, Lei;Li, Yuting;Su, Longxing;Li, Wei;Liu, Yanlin;Xu, Qiang;Jiang, Yunfeng;Jiang, Qiantao;Chen, Guoyue;Wei, Yuming;Ma, Jian;Chen, Guangdeng;Pu, Zhien;Jiang, Yun
关键词：
-
Genome editing of five starch synthesis genes produces highly resistant starch and dietary fibre in barley grains
作者：Yang, Qiang;Wei, Yuming;Zheng, Youliang;Jiang, Qiantao;Yang, Qiang;Yang, Qiang;Ral, Jean-Philippe;Li, Zhongyi
关键词：barley; multiplex genome editing; starch synthase; starch-branching enzyme; resistant starch; dietary fibre
-
A co-located QTL for seven spike architecture-related traits shows promising breeding use potential in common wheat (Triticum aestivum L.)
作者：Zhou, Jieguang;Liu, Qian;Tian, Rong;Yang, Yaoyao;Zhao, Conghao;Liu, Yanlin;Tang, Huaping;Deng, Mei;Xu, Qiang;Jiang, Qiantao;Chen, Guoyue;Qi, Pengfei;Jiang, Yunfeng;Zheng, Youliang;Wei, Yuming;Ma, Jian;Zhou, Jieguang;Liu, Qian;Tian, Rong;Chen, Huangxin;Wang, Jian;Yang, Yaoyao;Zhao, Conghao;Liu, Yanlin;Tang, Huaping;Deng, Mei;Xu, Qiang;Jiang, Qiantao;Chen, Guoyue;Qi, Pengfei;Jiang, Yunfeng;Zheng, Youliang;Wei, Yuming;Ma, Jian;Chen, Guangdeng;Tang, Liwei;Ren, Yong;He, Yuanjiang;Zheng, Zhi;Liu, Chunji
关键词：
-
Major and stably expressed QTL for traits related to the mature wheat embryo independent of kernel size
作者：Wang, Surong;Xuan, Qijing;Qu, Xiangru;Xu, Qiang;Jiang, Qiantao;Jiang, Yunfeng;Chen, Guoyue;Deng, Mei;Liu, Yanling;Tang, Huaping;Wei, Yuming;Zheng, Youliang;Ma, Jian;Wang, Surong;Xuan, Qijing;Qu, Xiangru;Xu, Qiang;Jiang, Qiantao;Jiang, Yunfeng;Chen, Guoyue;Deng, Mei;Liu, Yanling;Tang, Huaping;Wei, Yuming;Zheng, Youliang;Ma, Jian;Wang, Tianyu;Pu, Zhien;Li, Yang;Gou, Lulu;Chen, Guangdeng;He, Yuanjiang
关键词：
-
A major QTL simultaneously increases the number of spikelets per spike and thousand-kernel weight in a wheat line
作者：Zhao, Conghao;Zhou, Jieguang;Li, Cong;Liu, Yanling;Tang, Huaping;Deng, Mei;Xu, Qiang;Zhang, Yazhou;Jiang, Qiantao;Chen, Guoyue;Qi, Pengfei;Jiang, Yunfeng;Wang, Jirui;Zheng, Youliang;Wei, Yuming;Ma, Jian;Zhao, Conghao;Zhou, Jieguang;Li, Cong;Liu, Yanling;Tang, Huaping;Deng, Mei;Xu, Qiang;Zhang, Yazhou;Jiang, Qiantao;Chen, Guoyue;Qi, Pengfei;Jiang, Yunfeng;Wang, Jirui;Zheng, Youliang;Wei, Yuming;Ma, Jian;Li, Wei;Pu, Zhien;Chen, Guangdeng;Jiang, Yun;Zheng, Zhi;Liu, Chunji;You, Jianing
关键词：
-
A promising QTL QSns.sau-MC-3D.1 likely superior to WAPO1 for the number of spikelets per spike of wheat shows no adverse effects on yield-related traits
作者：Zhou, Jieguang;Li, Wei;Yang, Yaoyao;Xie, Xinlin;Liu, Jiajun;Liu, Yanling;Tang, Huaping;Deng, Mei;Xu, Qiang;Jiang, Qiantao;Chen, Guoyue;Qi, Pengfei;Jiang, Yunfeng;Zheng, Youliang;Wei, Yuming;Ma, Jian;Zhou, Jieguang;Li, Wei;Yang, Yaoyao;Xie, Xinlin;Liu, Jiajun;Liu, Yanling;Tang, Huaping;Deng, Mei;Xu, Qiang;Jiang, Qiantao;Chen, Guoyue;Qi, Pengfei;Jiang, Yunfeng;Zheng, Youliang;Wei, Yuming;Ma, Jian;Chen, Guangdeng;He, Yuanjiang;Ren, Yong;Tang, Liwei;Gou, Lulu
关键词：
