文献类型： 外文期刊

作者： Zhou, Kang ¹ ; Zhang, Fan ³ ; Deng, Yue ⁵ ;

作者机构： 1.Fuyang Normal Univ, Anhui Prov Key Lab Environm Hormone & Reprod, Fuyang 236037, Peoples R China

2.Fuyang Normal Univ, Anhui Prov Key Lab Embryo Dev & Reprod Regulat, Fuyang 236037, Peoples R China

3.Huazhong Agr Univ, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

4.Huazhong Agr Univ, Hubei Key Lab Plant Pathol, Wuhan 430070, Peoples R China

5.Sichuan Acad Agr Sci, Inst Plant Protect, Chengdu 610066, Peoples R China

6.Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Crops Southwest, Chengdu 610066, Peoples R China

关键词： macrofungi; virome; viral diversity; ambi-like viruses

期刊名称：VIRUSES-BASEL （ 影响因子：4.7； 五年影响因子：4.8 ）

ISSN：

年卷期： 2024 年 16 卷 4 期

页码：

收录情况： SCI

摘要： Macrofungi play important roles in the soil elemental cycle of terrestrial ecosystems. Fungal viruses are common in filamentous fungi, and some of them can affect the growth and development of hosts. However, the composition and evolution of macrofungal viruses are understudied. In this study, ninety strains of Trametes versicolor, Coprinellus micaceus, Amanita strobiliformis, and Trametes hirsuta were collected in China. Four mixed pools were generated by combining equal quantities of total RNA from each strain, according to the fungal species, and then subjected to RNA sequencing. The sequences were assembled, annotated, and then used for phylogenetic analysis. Twenty novel viruses or viral fragments were characterized from the four species of macrofungi. Based on the phylogenetic analysis, most of the viral contigs were classified into ten viral families or orders: Barnaviridae, Benyviridae, Botourmiaviridae, Deltaflexiviridae, Fusariviridae, Hypoviridae, Totiviridae, Mitoviridae, Mymonaviridae, and Bunyavirales. Of these, ambi-like viruses with circular genomes were widely distributed among the studied species. Furthermore, the number and overall abundance of viruses in these four species of macrofungi (Basidiomycota) were found to be much lower than those in broad-host phytopathogenic fungi (Ascomycota: Sclerotinia sclerotiorum, and Botrytis cinerea). By employing metatranscriptomic analysis in this study, for the first time, we demonstrated the presence of multiple mycoviruses in Amanita strobiliformis, Coprinellus micaceus, Trametes hirsute, and Trametes versicolor, significantly contributing to research on mycoviruses in macrofungi.