文献类型： 外文期刊
作者： Wen, Chun-Miao 2 ; Cai, Ping-Zhong 1 ; Xu, Zheng-Jun 2 ; Zhong, Wan-Fang 3 ;
作者机构： 1.Sichuan Acad Agr Sci, Inst Biotechnol & Nucl Technol, Chengdu 610066, Peoples R China
2.Sichuan Agr Univ, Rice Inst, Chengdu 611130, Peoples R China
3.Jaingsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China
关键词： intron-containing hairpin RNA;genomic DNA;recombinant-PCR;Zea mays;auxin-binding protein;DOUBLE-STRANDED-RNA;GENETIC INTERFERENCE;GENOMIC DNA;EXPRESSION
期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）
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收录情况： SCI
摘要： Recently, much research on constitutive expression of an intron-containing self-complementary hair-pin RNA (ihpRNA) have been reported to silence target genes efficiently in a variety of species. Here we designed a new recombinant-PCR mediated method called direct amplification of ihpRNA from genomic DNA. This approach has proved to be easy, stable, and efficient.
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关键词：Bacillus thuringiensis serovar sotto;chitinase;touchdown PCR;gene cloning;characterization analysis
