文献类型： 外文期刊
作者： Zhao, Wei 1 ; Zhou, Jian 2 ; Li, Zhe 1 ; Jing, Tingsen 1 ; Zhao, Li 1 ; Ye, Hua 1 ;
作者机构： 1.Southwest Univ, Coll Anim Sci, Chongqing 402460, Peoples R China
2.Sichuan Acad Agr Sci, Inst Fisheries, Chengdu 611731, Peoples R China
3.Southwest Univ, Fisheries Breeding & Hlth Cultivat Res Ctr, Chongqing 402460, Peoples R China
关键词： Leiocassis longirostris; Single nucleotide polymorphism (SNPs); Sequenom MassARRAY
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）
ISSN： 1877-7252
年卷期：
页码：
收录情况： SCI
摘要： The Chinese longsnout catfish (Leiocassis longirostris) is a commercially important aquaculture species in China. The germplasm resources of L. longirostris have been seriously degraded by human activities, therefore, it is very urgent to develop genetic markers of L. longirostris to investigate the natural resources. Here, a total of 55 single nucleotide polymorphism (SNP) loci were identified using Sequenom MassARRAY based on the L. longirostris transcriptome. Genetic parameters of the loci showed that the minor allele frequency varied from 0.0417 to 0.5000, and that the observed and expected heterozygosity ranged from 0.0833 to 0.8750 and 0.0802 to 0.5021, respectively. 27 loci were identified deviating significantly from the Hardy-Weinberg equilibrium (P < 0.05), and no linkage disequilibrium was found between pairs of loci. These SNP markers will be useful for future population conservation and construction of genetic linkage maps related to L. longirostris.
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