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Rapid Identification of Enhanced Drought and Salt Tolerance in Arabidopsis Conferred by BnBADH1 Gene

文献类型： 外文期刊

作者： Chai, Liang 1 ; Li, Haojie 1 ; Yang, Luhan 2 ; Zheng, Benchuan 1 ; Cui, Cheng 1 ; Jiang, Jun 1 ; Zhang, Jinfang 1 ; Jiang 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China

2.Chengdu Zhonghe High Sch, Chengdu 610212, Sichuan, Peoples R China

关键词： Abiotic stresses; Betaine aldehyde dehydrogenase; Brassica napus; Gene expression level; Transgenic plants

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2019 年 22 卷 4 期

页码：

收录情况： SCI

摘要： Identifying and applying salt or drought tolerant genes is a quick method of enhancing crop tolerance to environmental stresses. In this study, a full-length new betaine aldehyde dehydrogenase gene from Brassica napus (BnBADH1) was isolated by homologous cloning method according to the sequence of Arabidopsis BADH1 gene. BnBADH1 was 1506 bp in length and it theoretically encoded a hypothetical protein with 501 aa; sharing 90.2% identity with its ortholog in Arabidopsis (GenBank: ALDH10A8) in nucleotide sequence and 93.4% identity in amino acid sequence. The mRNA expression levels of BnBADH1 under drought or salt stress was investigated by quantitative real-time PCR. Under drought stress, the expression level of BnBADH1 mRNA began to decrease slightly from 12 h to 72 h; but it suddenly increased at 96 h and reached a peak at 120 h; after that, its expression reduced drastically from 144 h to 192 h. While under salt stress, there was no obvious difference in expression level at the first 8 h (2-8 h); but mRNA increased sharply at 20 h. Moreover, the BnBADH1 gene was induced into Arabidopsis and overexpressed. Drought and salt stress tolerance was enhanced in Arabidopsis as was expressed by higher root length of transgenic plants compared with its wild plants. It is the first report which highlighted the expressions of newly isolated gene BnBADH1 from rapeseed in transgenic Arabidopsis to enhance drought and salt stresses tolerance. (C) 2019 Friends Science Publishers

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