Development of 'multiresistance rice' by an assembly of herbicide, insect and disease resistance genes with a transgene stacking system
文献类型： 外文期刊
作者： Li, Chuanxu 1 ; Zhang, Jianguo 1 ; Ren, Zhiyong 3 ; Xie, Rong 4 ; Yin, Changxi 2 ; Ma, Weihua 2 ; Zhou, Fei 1 ; Chen, Hao 1 ;
作者机构： 1.Natl Ctr Plant Gene Res, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
2.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Hubei, Peoples R China
3.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan, Peoples R China
4.Sichuan Acad Agr Sci, Natl Rice Improvement Ctr, Key Lab Southwest Rice Biol & Genet Breeding, Rice & Sorghum Res Inst,Minist Agr,Luzhou Branch, Deyang, Peoples R China
关键词： rice; herbicide; borer; brown planthopper; bacterial blight; rice blast; multigene transformation; pest management
期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）
ISSN： 1526-498X
年卷期： 2021 年 77 卷 3 期
页码：
收录情况： SCI
摘要： Background Weeds, diseases and pests pose serious threats to rice production and cause significant economic losses. Cultivation of rice varieties with resistance to herbicides, diseases and pests is believed to be the most economical and environmentally friendly method to deal with these problems. Results In this study, a highly efficient transgene stacking system was used to assembly the synthetic glyphosate-tolerance gene (I. variabilis-EPSPS*), lepidopteran pest resistance gene (Cry1C*), brown planthopper resistance genes (Bph14* and OsLecRK1*), bacterial blight resistance gene (Xa23*) and rice blast resistance gene (Pi9*) onto a transformable artificial chromosome vector. The construct was transferred into ZH11 (a widely used japonica rice cultivar Zhonghua 11) via Agrobacterium-mediated transformation and 'multiresistance rice' (MRR) with desirable agronomic traits was obtained. The results showed that MRR had significantly improved resistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast relative to the recipient cultivar ZH11. Besides, under the natural occurrence of pests and diseases in the field, the yield of MRR was significantly higher than that of ZH11. Conclusion A multigene transformation strategy was employed to successfully develop rice lines with multiresistance to glyphosate, borers, brown planthopper, bacterial blight and rice blast, and the obtained MRR is expected to have great application potential.
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