An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae
文献类型： 外文期刊
作者： Li, Guangrong 1 ; Zhang, Tao 2 ; Yu, Zhihui 1 ; Wang, Hongjin 1 ; Yang, Ennian 4 ; Yang, Zujun 1 ;
作者机构： 1.Univ Elect & Technol China, Sch Life Sci & Technol, Ctr Informat Biol, Chengdu 611731, Peoples R China
2.Yangzhou Univ, Agr Coll, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Jiangsu Key Lab Crop Genom & Mol Breeding,Jiangsu, Yangzhou 225009, Jiangsu, Peoples R China
3.Yangzhou Univ, Minist Educ China, Joint Int Res Lab Agr & Agriprod Safety, Minist Educ,Key Lab Plant Funct Genom, Yangzhou 225009, Jiangsu, Peoples R China
4.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China
关键词： chromosome rearrangement; Oligo‐ FISH; Triticeae species; wheat‐ barley synteny
期刊名称：PLANT JOURNAL （ 影响因子：6.417； 五年影响因子：7.627 ）
ISSN： 0960-7412
年卷期： 2021 年 105 卷 4 期
页码：
收录情况： SCI
摘要： A chromosome-specific painting technique has been developed which combines the most recent approaches of the companion disciplines of molecular cytogenetics and genome research. We developed seven oligonucleotide (oligo) pools derivd from single-copy sequences on chromosomes 1 to 7 of barley (Hordeum vulgare L.) and corresponding collinear regions of wheat (Triticum aestivum L.). The seven groups of pooled oligos comprised between 10 986 and 12 496 45-bp monomers, and these then produced stable fluorescence in situ hybridization (FISH) signals on chromosomes of each linkage group of wheat and barley. The pooled oligo probes were applied to high-throughput karyotyping of the chromosomes of other Triticeae species in the genera Secale, Aegilops, Thinopyrum, and Dasypyrum, and the study also extended to some wheat-alien amphiploids and derived lines. We demonstrated that a complete set of whole-chromosome oligo painting probes facilitated the study of inter-species chromosome homologous relationships and visualized non-homologous chromosomal rearrangements in Triticeae species and some wheat-alien species derivatives. When combined with other non-denaturing FISH procedures using tandem-repeat oligos, the newly developed oligo painting techniques provide an efficient tool for the study of chromosome structure, organization, and evolution among any wild Triticeae species with non-sequenced genomes.
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