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Differential transcriptomic analysis of crayfish (Procambarus clarkii) from a rice coculture system challenged by Vibrio parahaemolyticus

文献类型： 外文期刊

作者： Zhou, Jian 1 ; Zhao, Han 1 ; Huang, Zhipeng 1 ; Ye, Xianlin 2 ; Zhang, Lu 1 ; Li, Qiang 1 ; Zhao, Zhongmeng 1 ; Su, XuTao 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Fisheries Inst, Chengdu 611731, Sichuan, Peoples R China

2.Sichuan Acad Agr Sci, Chengdu 610066, Sichuan, Peoples R China

关键词： Rice-crayfish coculture system; Vibrio parahaemolyticus; Immune response; mRNA sequencing

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS （ 影响因子：2.674； 五年影响因子：2.941 ）

ISSN： 1744-117X

年卷期： 2020 年 36 卷

页码：

收录情况： SCI

摘要： Rice-crayfish (Procambarus clarkii) coculture is an effective farming mode and has been promoted in various regions of China. However, infection in crayfish can be a significant economic drain. We found crayfish infected with Vibrio parahemolyticus (VP), and to understand the molecular mechanisms of the immune responses of crayfish to VP infection, Illumina sequencing was employed to identify changes in the mRNA of hepatopan-creatic tissue. A total of 47.30 and 43.01million high-quality transcriptome reads were generated from the hepatopancreatic samples of the experimental group (EG) and control group (CG), respectively. We found 5559 genes were significantly differentially expressed, including 2521 up-regulated genes (45.35%) and 3038 down regulated genes (54.65%). These genes were enriched in 126 GO terms and 76 KEGG pathways (P <= 0.05), including the MAPK and PI3K-Akt signaling pathways and cell adhesion molecules, with 23 up-regulated genes and 3 down-regulated genes related to immune responses in the EG relative to the CG. Histopathological analysis revealed that the epithelial cells of the hepatopancreatic tubules in the EG were severely atrophic, necrotic, and exfoliated, resulting in thin and collapsing hepatopancreatic tubules. The expression patterns of 8 differentially expressed genes involved in immune responses were validated by quantitative real-time RT-PCR. These results provide a valuable basis for the immune responses of crayfish to acute hepatopancreatic necrosis disease at transcriptome level.

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