Comparative analysis of transcriptome and metabolome explores the underlying mechanism of pod color variation in pea (Pisum sativum L.)
文献类型： 外文期刊
作者： Zhong, Xiaojuan 1 ; Yang, Mei 1 ; Zhang, Xiaoyan 2 ; Fan, Yuanfang 1 ; Wang, Xianshu 1 ; Xiang, Chao 1 ;
作者机构： 1.Sichuan Acad Agr Sci, Environm Friendly Crop Germplasm Innovat & Genet I, Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China
2.Qingdao Acad Agr Sci, Qingdao 266100, Shandong, Peoples R China
关键词： Pea pod; Color variation; Metabolome; Transcriptome; Flavonoid metabolism
期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY （ 影响因子：1.9； 五年影响因子：1.6 ）
ISSN： 0971-7811
年卷期： 2024 年 33 卷 2 期
页码：
收录情况： SCI
摘要： The pea (Pisum sativum L.) is one of widely cultivated crops over the world, but the mechanism of pod coloration is still unclear. Understanding the underlying mechanism regulating pea pod coloration can broaden pea breeding. In this study, the comparative analysis of transcriptome and metabolome was performed to examine the changes in metabolites accumulation and gene expression at two pod developmental stages in two pea accessions with green pods (GP) and yellow pods (YP). At the two omics levels, a total of 4504 differential metabolites (DEMs) were detected in four samples, and 2709 and 3725 differential expression genes (DEGs) were detected at two pod developmental stages between GP and YP, respectively. The verification results of RT-qPCR showed that the relative expression of ten genes randomly detected showed statistically high correlations between RT-qPCR and RNA-seq. The functional analysis of DEMs and DEGs found that the flavonoid metabolism pathway was the most direct metabolic pathway for the variation of pod coloration between two pea accessions. The 34 DEMs and 21 DEGs related to flavonoid metabolism pathway exhibited different expression pattern between GP and YP samples. Additionally, the combined analysis of transcriptome and metabolome in flavonoid biosynthesis pathway revealed that a direct correlation between the decreased expression level of genes and reduction accumulation of metabolites in the delphinidin biosynthesis pathway. The obtained results of this study provided a foundation to reveal the candidate genes controlling the pod color variation, and provide new germplasm resources for genetic improvement in pea.
- 相关文献
作者其他论文 更多>>
-
Metabolomic and Transcriptomic Analyses of Flavonoid Biosynthesis in Different Colors of Soybean Seed Coats
作者：Fan, Yuanfang;Wang, Xianshu;Yang, Mei;Zhong, Xiaojuan;Zhou, Yonghang;Xiang, Chao;Fan, Yuanfang;Wang, Xianshu;Yang, Mei;Zhong, Xiaojuan;Zhou, Yonghang;Xiang, Chao;Hussain, Sajad;Tao, Lei;Li, Jing
关键词：soybean; flavonoid; transcriptome; metabolome; seed coat color
-
Metabolomics and Transcriptomics Jointly Explore the Mechanism of Pod Color Variation in Purple Pod Pea
作者：Zhong, Xiaojuan;Yang, Mei;Fan, Yuanfang;Wang, Xianshu;Xiang, Chao;Zhang, Xiaoyan
关键词：
Pisum sativum L.; pod color; metabolome; transcriptome; flavonoid metabolism; anthocyanin -
QTL Mapping and Developing KASP Markers for High-Temperature Adult-Plant Resistance to Stripe Rust in Argentinian Spring Wheat William Som (PI 184597)
作者：Upadhaya, Arjun;Wang, Meinan;Xiang, Chao;Fatima, Nosheen;Ruff, Travis;See, Deven R.;Chen, Xianming;Xiang, Chao;Rynearson, Sheri;Pumphrey, Michael;Ruff, Travis;See, Deven R.;Chen, Xianming
关键词：high-temperature and adult plant resistance; KASP markers; quantitative trait loci; stripe rust; wheat
-
Quantifying high-temperature-induced reproductive growth imbalance in citrus at anthesis: Insights from the CF-ASPM model
作者：Qiu, Xia;Zhu, Feiyu;Huang, Ping;Chen, Hongwen;Li, Yali;Pu, Changbing;Li, Zongnan;Zhong, Dan;Wang, Si;Qiu, Xia;Zhu, Feiyu;Huang, Ping;Chen, Hongwen;Li, Yali;Pu, Changbing;Li, Zongnan;Zhong, Dan;Wang, Si;Xiang, Chao;Chen, Jin
关键词：Citrus flower; High-temperature stress; Quantitative phenotype; CF-ASPM; Reproductive growth imbalance
-
A special short-wing petal faba genome and genetic dissection of floral and yield-related traits accelerate breeding and improvement of faba bean
作者：Liu, Rong;Li, Mengwei;Shu, Qin;Wang, Zonghe;Zong, Xuxiao;Yang, Tao;Hu, Chaoqin;Yang, Xin;Dai, Zhengming;Yu, Haitian;Yang, Feng;Zheng, Aiqing;Lv, Meiyuan;He, Yuhua;Gao, Dan;Chen, Liyang;Jiao, Chengzhi;Yuan, Xingxing;Chen, Xin;Garg, Vanika;Chitikineni, Annapurna;Barmukh, Rutwik;Varshney, Rajeev K.;Zhang, Hongyan;Hou, Wanwei;Teng, Changcai;Zhou, Xianli;Liu, Yujiao;Zhang, Hongyan;Hou, Wanwei;Teng, Changcai;Zhou, Xianli;Du, Chengzhang;Xiang, Chao;Xu, Dongxu;Tang, Yongsheng;Duan, Yinmei;Maalouf, Fouad;Agrawal, Shiv Kumar;Wei, Libin;Zhao, Na;Li, Xiang;Wang, Dong;Ding, Hanfeng
关键词：Faba bean; Short-wing petal; Genome assembly; Genomic diversity; GWAS; Yield-related loci; Breeding; Improvement
-
Performance of Different Varieties of Spring Field Pea (Pisum sativum L.) under Irrigated and Rainfed Environments in North China
作者：Min, Gengmei;Zhang, Lijuan;Gou, Zhiwen;Yang, Jingyi;He, Yuhua;Zong, Xuxiao;Lian, Rongfang;Liu, Zhenxing;Xiang, Chao;Li, Ling;Xing, Baolong
关键词：multi-environment yield trials; pea; genotypes; grain yield; adaptability
-
Disease Resistance and Molecular Variations in Irradiation Induced Mutants of Two Pea Cultivars
作者：Deng, Dong;Sun, Suli;Wu, Wenqi;Duan, Canxing;Zhu, Zhendong;Deng, Dong;Wu, Xuehong;Xiang, Chao;Yu, Dongmei
关键词：Pisum sativum; mutant; disease resistance; genetic variation; er1-2
