文献类型: 外文期刊
作者: Liu, Peixun 1 ; Ma, Xiaofei 2 ; Wan, Hongshen 1 ; Zheng, Jianmin 1 ; Luo, Jiangtao 1 ; Hu, Yuanbin 3 ; Pu, Zongjun 1 ;
作者机构: 1.Sichuan Acad Agr Sci, Green Crop Germplasm Innovat & Genet Improvement, Key Lab Wheat Biol & Genet Improvement SouthWeste, Minist Agr & Rural Affairs,Crop Res Inst, Chengdu 610066, Sichuan, Peoples R China
2.Shanxi Agr Univ, Wheat Res Inst, Linfen 041000, Shanxi, Peoples R China
3.Sichuan Acad Anim Sci, Chengdu 610066, Sichuan, Peoples R China
关键词: Grain hardness; Nitrogen application; Tandem mass tag (TMT); Proteomics
期刊名称:JOURNAL OF CEREAL SCIENCE ( 影响因子:3.616; 五年影响因子:3.891 )
ISSN: 0733-5210
年卷期: 2021 年 102 卷
页码:
收录情况: SCI
摘要: Wheat, an important human energy source, is critical for global food security. Grain hardness is a major determinant of wheat quality. Although the genetic bases of wheat grain hardness have been investigated, the mechanisms underlying the effects of nitrogen on wheat quality remain largely unknown. In the same wheat cultivar, grain hardness and protein content decreased in plants treated with low nitrogen as compared to those treated with high nitrogen, while starch content increased. High-throughput tandem mass tags (TMT) quantitative proteomics identified 6061 effective proteins in cultivar Shumai 969; 88 of these proteins were differentially expressed in the low-nitrogen plants as compared to the high-nitrogen plants (31 upregulated and 57 downregulated). Several storage proteins, including alpha-gliadins, gamma-gliadins, ro-gliadins, high molecular weight glutenin subunits, and three globulin proteins, were downregulated in the low-nitrogen plants as compared to the high-nitrogen plants. The differentially expressed proteins associated with the 10 significantly enriched Gene Ontology terms and the 5 significantly enriched biochemical metabolic pathways might play a role in the determination of wheat grain hardness. This study provides a baseline for the effects of nitrogen application on wheat grain quality and identifies candidate genes for the targeted breeding of superior wheat varieties with specific end-use properties.
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