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The localization of the alkaloids in Coptis chinensis rhizome by time-of-flight secondary ion mass spectrometry

文献类型： 外文期刊

作者： He, Fan 1 ; Huang, Yu-Feng 1 ; Dai, Wei 2 ; Qu, Xian-You 3 ; Lu, Jing-Guang 4 ; Lao, Chi-Chou 4 ; Luo, Wen-Hui 5 ; Sun, Dong-Mei 5 ; Wei, Mei 5 ; Xiao, Sheng-Yuan 6 ; Xie, Ying 1 ; Liu, Liang 1 ; Zhou, Hua 1 ;

作者机构： 1.Guangdong Prov Hosp Chinese Med, Guangdong Prov Acad Chinese Med Sci, Guangzhou, Guangdong, Peoples R China

2.Inst Chinese Med Mat, Mianyang Acad Agr Sci, Mianyang, Sichuan, Peoples R China

3.Chongqing Acad Chinese Mat Med, Chongqing Key Lab Tradit Chinese Resources, Chongqing, Peoples R China

4.Macau Univ Sci & Technol, Fac Chinese Med, State Key Lab Qual Res Chinese Med, Taipa, Macao, Peoples R China

5.Guangdong Yifang Pharmaceut Co Ltd, Guangdong Prov Key Lab Tradit Chinese Med Formula, Foshan, Guangdong, Peoples R China

6.Jilin Agr Univ, Coll Chinese Med Mat, Changchun, Jilin, Peoples R China

关键词： Coptis rhizome; TOF-SIMS; UPLC-QQQ-MS; MS; alkaloids; localization

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： BackgroundUnderstanding the spatial distribution of active compounds can effectively evaluate the quality of decoction pieces of traditional Chinese medicine (TCM). Traditional methods are economical and practical but lack chemical information on the original distribution. Time-of-flight secondary ion mass spectrometry (TOF-SIMS), with the advantage of non-destructive detection of samples, can directly analyze the distribution of chemical compounds on the surface of various samples. MethodsIn this study, TOF-SIMS image analysis technology was used to detect TCM for the first time. Taking Coptis rhizome (CR) as an example, a commonly used TCM, the distribution of the compounds in the cross-section of CR was studied. Meanwhile, ultra-high-performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLCQQQ-MS/MS) was used to verify the results of TOF-SIMS. ResultsThe distribution of nine active compounds: berberine, epiberberine, coptisine, palmatine, columbamine, jatrorrhizine, tetrahydricheilanthifolinium, and oxyberberine, was well imaged in the cross-section of CR by TOF-SIMS. The content of berberine and epiberberine was the highest; Palmatine distribution in the pith was more than that in other parts; Oxyberberine was mainly concentrated in the cork and xylem rays. Normalization analysis showed contents of these compounds increased along with the growth years. The result was consistent with UPLC-QQQ-MS/MS. ConclusionThe TOF-SIMS method can display the spatial distribution status of the active compounds of herbs, providing a basis for selecting the medicine site with non-destructive and fast detection.

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