文献类型： 外文期刊

作者： Xu, Tengfei ¹ ; Gao, Dehang ³ ; Wu, Mengmeng ³ ; Wang, Hongqing ³ ; He, Chengyong ¹ ;

作者机构： 1.Sichuan Acad Agr Sci, Inst Hort, Chengdu 610000, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Hort Crops Biol & Germplasm Enhancement So, Chengdu 610000, Peoples R China

3.China Agr Univ, Coll Hort, Dept Fruit Sci, Beijing 100107, Peoples R China

关键词： SMoV; SVBV; detection system; TaqMan RT-qPCR

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 15 期

页码：

收录情况： SCI

摘要： SMoV and SVBV are two major viruses that pose significant threats to the global strawberry industry. Both are latent viruses, making early detection difficult due to their uneven distribution and low concentration in host tissues. Traditional RT-PCR techniques are insufficient for precise and quantitative detection. In this study, TaqMan RT-qPCR detection systems for SMoV and SVBV were established for application in practical production settings, enabling accurate, rapid, and efficient detection of strawberry viruses. When viral accumulation in plants is low, the highly sensitive TaqMan RT-qPCR technique allows for accurate quantification, facilitating the early identification of infected plants and preventing large-scale outbreaks in cultivation areas. The development of a duplex TaqMan RT-qPCR assay enables simultaneous quantification of SMoV and SVBV in a single reaction, improving detection efficiency and providing technical support for risk assessment and effective control of strawberry viral diseases.