First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha
文献类型： 外文期刊
作者： Sekete, Malota 2 ; Ma, Duanzheng 1 ; Wang, Bo 3 ; Wang, Hexiang 1 ; Ng, Tzibun 4 ;
作者机构： 1.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China
2.China Agr Univ, Dept Microbiol, Beijing 100193, Peoples R China
3.Sichuan Acad Agr Sci, Soil & Fertilizer Inst, Chengdu 610066, Sichuan, Peoples R China
4.Chinese Univ Hong Kong, Fac Med, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
关键词： Ribonuclease; Mushroom; Purification
期刊名称：SPRINGERPLUS （ 影响因子：1.13； 五年影响因子：1.29 ）
ISSN： 2193-1801
年卷期： 2012 年 1 卷
页码：
收录情况： SCI
摘要： A 45-kDa ribonuclease (RNase) was purified from dried fruiting bodies of the wild mushroom Amanita hemibapha. It was adsorbed on DEAE-cellulose, S-sepharose, and finally purified on Superdex 75. The RNase exhibited maximal RNase activity at pH 5 and in a temperature range between 60-70 degrees C. It demonstrated no ribonucleolytic activity toward four polyhomoribonucleotides. The amino acid sequence analysis (GDDETFWEHEWAK) showed this RNase was a ribonuclease T2-like RNase. It exhibited strong inhibitory activity against HIV-1 reverse transcriptase (HIV-1 RT) with an IC50 of 17 mu M.
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