文献类型： 外文期刊

作者： Su, Siyuan ¹ ; Ding, Xiang ³ ; Hou, Yiling ¹ ; Liu, Binbin ² ; Du, Zhouhe ² ; Liu, Junfeng ² ;

作者机构： 1.China West Normal Univ, Coll Life Sci, Key Lab Southwest China Wildlife Resources Conserv, Nanchong 637009, Peoples R China

2.Sichuan Acad Agr Sci, Sericultural Res Inst, Nanchong 637000, Peoples R China

3.China West Normal Univ, Coll Environm Sci & Engn, Nanchong 637009, Peoples R China

关键词： Boletus reticulatus Schaeff; Polysaccharides; Structure identification; Immunomodulatory activity; Antitumor activity

期刊名称：FOOD SCIENCE AND HUMAN WELLNESS （ 影响因子：7.0； 五年影响因子：8.3 ）

ISSN：

年卷期： 2023 年 12 卷 2 期

页码：

收录情况： SCI

摘要： A new water-soluble heteropolysaccharide with a molecular weight of 15 kDa was isolated from the fruiting bodies of Boletus reticulatus Schaeff. Structural characterization results revealed that B. reticulatus Schaeff polysaccharide (BRS-X) had a backbone of 1,6-linked alpha-D-galactose and 1,2,6-linked alpha-D-galactose which branches were mainly composed of a terminal 4-linked beta-D-glucose and the ratio of D-galactose and D-glucose was 5:1. Bioactivity assays indicated that BRS-X displayed a strong proliferative activity in T cells and B cells and promoted the secretion of immunoglobulin G (IgG), IgE, IgD and IgM. In addition, BRS-X could facilitate the proliferation and phagocytosis of RAW264.7 cells and could significantly inhibit the growth of tumors in S180-bearing mice. The results of transcriptome sequencing analysis illustrated that total 46 genes enriched in MAPK and total 34 genes enriched in PI3K/Akt signaling pathways in BRS-X group. The protein VEGF and VEGFR expression were significantly reduced under the treatment with BRS-X. These findings provide a scientifi c basis for the edible and medicinal value of BRS-X.(c) 2023 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAiCommunications Co., Ltd. This is an open access article under the CC BY-NC-ND license(http://creativecommons.org/licenses/by-nc-nd/4.0/).